An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Diogo Borges Lima
Department of Chemical Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6056-0825
Julia Chamot-Rooke
Mass Spectrometry for Biology Unit, CNRS USR 2000, Institut Pasteur, Paris, France
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9427-543X
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a new software-tool for visualizing fragment ions and sequence coverage of intact proteins in top-down mass spectrometry. TDFragMapper combines the data arising from multiple and diverse tandem mass spectrometry experiments of intact proteins. Our tool maps fragment ions onto the protein backbone sequence and allows for a rapid comparison of the results obtained when varying experimental parameters in tandem mass spectrometry (MS/MS) experiments of intact proteins: fragmentation method, selected precursor charge state, activation level, technical replicate. In summary, TDFragMapper is a unique software-tool that allows an easy selection of the best experimental conditions leading to the highest confidence in intact protein sequence identification.
Keywords
Bioinformatics, Proteomics, Top-Down, Mass Spectrometry, Intact Proteins
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 May, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Diogo Borges Lima
Department of Chemical Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Berlin, Germany
Corresponding Author
ORCiD: https://orcid.org/0000-0001-6056-0825
Julia Chamot-Rooke
Mass Spectrometry for Biology Unit, CNRS USR 2000, Institut Pasteur, Paris, France
Corresponding Author
ORCiD: https://orcid.org/0000-0002-9427-543X
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 May, 2021
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we present a new software-tool for visualizing fragment ions and sequence coverage of intact proteins in top-down mass spectrometry. TDFragMapper combines the data arising from multiple and diverse tandem mass spectrometry experiments of intact proteins. Our tool maps fragment ions onto the protein backbone sequence and allows for a rapid comparison of the results obtained when varying experimental parameters in tandem mass spectrometry (MS/MS) experiments of intact proteins: fragmentation method, selected precursor charge state, activation level, technical replicate. In summary, TDFragMapper is a unique software-tool that allows an easy selection of the best experimental conditions leading to the highest confidence in intact protein sequence identification.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Loading...