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Method Article
Laura Dioni
EPIGET Lab, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In this protocol, we describe a method to investigate the presence of SARS-CoV-2 RNA in nasal swabs, using a commercially available high-throughput Real-Time Polymerase Chain Reaction (RT-PCR) assay (TaqPath™ Covid-19 kit, ThermoFisher Scientific) in a 384-Well Plate. After Viral RNA extraction with QIAamp Viral RNA Mini kit, reverse transcription and cDNA amplification, all samples are assessed for the presence of three specific SARS-CoV-2 viral genomic regions and an internal positive control (IPC), in one Multiplex RT-PCR reaction.
Keywords
Swab, SARS-CoV-2 RNA, high-throughput Multiplex RT-PCR
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Version 1
Posted 10 Nov, 2020
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Subject Areas
Molecular biology
Method Article
Laura Dioni
EPIGET Lab, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 10 Nov, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
In this protocol, we describe a method to investigate the presence of SARS-CoV-2 RNA in nasal swabs, using a commercially available high-throughput Real-Time Polymerase Chain Reaction (RT-PCR) assay (TaqPath™ Covid-19 kit, ThermoFisher Scientific) in a 384-Well Plate. After Viral RNA extraction with QIAamp Viral RNA Mini kit, reverse transcription and cDNA amplification, all samples are assessed for the presence of three specific SARS-CoV-2 viral genomic regions and an internal positive control (IPC), in one Multiplex RT-PCR reaction.
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