This is a protocol preprint. Preprints are preliminary reports that have not undergone peer review. They should not be considered conclusive, used to inform clinical practice, or referenced by the media as validated information.
Method Article
SARS-CoV-2 RNA detection in nasal mid-turbinate swabs
Laura Dioni
EPIGET Lab, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
In this protocol, we describe a method to investigate the presence of SARS-CoV-2 RNA in nasal swabs, using a commercially available high-throughput Real-Time Polymerase Chain Reaction (RT-PCR) assay (TaqPath™ Covid-19 kit, ThermoFisher Scientific) in a 384-Well Plate. After Viral RNA extraction with QIAamp Viral RNA Mini kit, reverse transcription and cDNA amplification, all samples are assessed for the presence of three specific SARS-CoV-2 viral genomic regions and an internal positive control (IPC), in one Multiplex RT-PCR reaction.
Keywords
Swab, SARS-CoV-2 RNA, high-throughput Multiplex RT-PCR
Version History
CURRENT STATUS: Posted
Version 1
Posted 10 Nov, 2020
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Subject Areas
Molecular biology
Method Article
SARS-CoV-2 RNA detection in nasal mid-turbinate swabs
Laura Dioni
EPIGET Lab, Department of Clinical Sciences and Community Health, Università degli Studi di Milano, Milan, Italy
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 10 Nov, 2020
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Declarations:
In this protocol, we describe a method to investigate the presence of SARS-CoV-2 RNA in nasal swabs, using a commercially available high-throughput Real-Time Polymerase Chain Reaction (RT-PCR) assay (TaqPath™ Covid-19 kit, ThermoFisher Scientific) in a 384-Well Plate. After Viral RNA extraction with QIAamp Viral RNA Mini kit, reverse transcription and cDNA amplification, all samples are assessed for the presence of three specific SARS-CoV-2 viral genomic regions and an internal positive control (IPC), in one Multiplex RT-PCR reaction.