Cultured chromaffin cells are a well established model for neurosecretion studies. They are embryologically derived from neural crest as symphathetic neurons and they have been extensively used as neuronal model and considered as paraneurons.
Both poor yield and short survival of cells are still well known problems to people working with chromaffin cells.
Small rodent animals, in particular rats, are becoming a popular model due to limitations to obtain bovine tissues after spongiform encephalopathy crisis or if you are interested in cells from breed controlled or genetically-defined animals.
We propose a thoroughly tested protocol to be used as reference to help standardization of both the isolation procedure and the quality of the cultures allowing a better comparison of the results obtained from different laboratories.