Purification and crystallization of Complement C3b

doi:10.1038/nprot.2006.416

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Method Article

Abdul Ajees Abdul Salam, K. Gunasekaran, John E. Volanakis, S.V.L. Narayana, Girish J. Kotwal, H.M. Krishna Murthy

Abdul Ajees Abdul Salam

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Corresponding Author

K. Gunasekaran

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

John E. Volanakis

Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

S.V.L. Narayana

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Girish J. Kotwal

Division of Medical Virology, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, HSC, Cape Town, South Africa, 7925

H.M. Krishna Murthy

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

DOI:

10.1038/nprot.2006.416

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Associated Publications

The structure of complement C3b provides insights into complement activation and regulation
by Ajees, A.A. et al.
Nature (24 August, 2006)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Abdul Ajees Abdul Salam, K. Gunasekaran, John E. Volanakis, S.V.L. Narayana, Girish J. Kotwal, H.M. Krishna Murthy

Abdul Ajees Abdul Salam

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Corresponding Author

K. Gunasekaran

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

John E. Volanakis

Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

S.V.L. Narayana

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Girish J. Kotwal

Division of Medical Virology, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, HSC, Cape Town, South Africa, 7925

H.M. Krishna Murthy

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Version History

CURRENT STATUS: Posted

Version 1

Posted 14 Dec, 2006

Community comments: 2

MetricsComments: 0HTML Views: ...

DOI:

10.1038/nprot.2006.416

Download PDF

The full text of this article is available to read as a PDF.

Associated Publications

The structure of complement C3b provides insights into complement activation and regulation
by Ajees, A.A. et al.
Nature (24 August, 2006)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Abdul Ajees Abdul Salam, K. Gunasekaran, John E. Volanakis, S.V.L. Narayana, Girish J. Kotwal, H.M. Krishna Murthy

Abdul Ajees Abdul Salam

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Corresponding Author

K. Gunasekaran

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

John E. Volanakis

Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

S.V.L. Narayana

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Girish J. Kotwal

Division of Medical Virology, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, HSC, Cape Town, South Africa, 7925

H.M. Krishna Murthy

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

DOI:

10.1038/nprot.2006.416

Download PDF

Introduction

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CURRENT STATUS: Posted

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Associated Publications

The structure of complement C3b provides insights into complement activation and regulation

by Ajees, A.A. et al.

Nature (24 August, 2006)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Abdul Ajees Abdul Salam, K. Gunasekaran, John E. Volanakis, S.V.L. Narayana, Girish J. Kotwal, H.M. Krishna Murthy

Abdul Ajees Abdul Salam

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Corresponding Author

K. Gunasekaran

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

John E. Volanakis

Department of Medicine, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

S.V.L. Narayana

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

Girish J. Kotwal

Division of Medical Virology, Institute for Infectious Diseases and Molecular Medicine, University of Cape Town, HSC, Cape Town, South Africa, 7925

H.M. Krishna Murthy

Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 1530 3rd Avenue South, Birmingham AL, 35294

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DOI:

10.1038/nprot.2006.416

Download PDF

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Declarations

Introduction

Reagents

Equipment

Procedure

Timing

Critical Steps

References