To produce our target nanozyme with a narrow size distribution, solvothermal method was performed to composite iron sulfide at high temperatures and pressures. Here we describe the protocol for synthesizing Cys-nFeS.
Method Article
Protocol for the synthesis of Cys-nFeS
https://doi.org/10.1038/protex.2018.137
This work is licensed under a CC BY 4.0 License
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted 03 Dec, 2018
You are reading this latest protocol version
To produce our target nanozyme with a narrow size distribution, solvothermal method was performed to composite iron sulfide at high temperatures and pressures. Here we describe the protocol for synthesizing Cys-nFeS.
To produce inorganic polysulfide from organosulfur compound, solvothermal method was performed to prepare nano-iron sulfide at high temperature and pressure in a sealed autoclave reactor1,2. Here we describe the protocol for synthesizing Cys-nFeS.
ethylene glycol, FeCl3•6H2O, Sodium acetate(NaOAc), cysteine
magnetic stirring bar, oven, Teflon-lined stainless steel autoclave
14 h or longer(including the mixing process and the reaction time under high temperature and high pressure)
The new formed products are black powder which consist of hexagonal sheet-like nanostructures and sphere nanoparticles characterized by electron microscope.
1.Gao, L. et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2, 577-583(2007).
2.Gao, L. Z. et al. Magnetite Nanoparticle-Linked Immunosorbent Assay. J Phys Chem C 112, 17357-17361(2008).
3.Fan, K. et al. Optimization of Fe3O4 nanozyme activity via single amino acid modification mimicking an enzyme active site.Chem Commun 53, 424-427(2016).
The authors declare no conflicting financial interests.
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted 03 Dec, 2018
You are reading this latest protocol version
© Research Square 2022