To produce our target nanozyme with a narrow size distribution, solvothermal method was performed to composite iron sulfide at high temperatures and pressures. Here we describe the protocol for synthesizing Cys-nFeS.
Iron oxide, nanoparticles, cysteine, solvothermal
To produce inorganic polysulfide from organosulfur compound, solvothermal method was performed to prepare nano-iron sulfide at high temperature and pressure in a sealed autoclave reactor1,2. Here we describe the protocol for synthesizing Cys-nFeS.
ethylene glycol, FeCl3•6H2O, Sodium acetate(NaOAc), cysteine
magnetic stirring bar, oven, Teflon-lined stainless steel autoclave
14 h or longer(including the mixing process and the reaction time under high temperature and high pressure)
The new formed products are black powder which consist of hexagonal sheet-like nanostructures and sphere nanoparticles characterized by electron microscope.
1.Gao, L. et al. Intrinsic peroxidase-like activity of ferromagnetic nanoparticles. Nat Nanotechnol 2, 577-583(2007).
2.Gao, L. Z. et al. Magnetite Nanoparticle-Linked Immunosorbent Assay. J Phys Chem C 112, 17357-17361(2008).
3.Fan, K. et al. Optimization of Fe3O4 nanozyme activity via single amino acid modification mimicking an enzyme active site.Chem Commun 53, 424-427(2016).