Jurkat Cells, Clone E6-1 ATCC, Manassas, VA TIB¬-152
NFAT-GFP-Jurkat Cells Provided by Arthur Weiss and Yvonne Chen N/A
K562-A2.1+ cells ATCC, Manassas, VA CCL-243
GXR-B27+ cells Provided by Bruce D. Walker N/A
Primary T Cells UCLA, CFAR Virology Core N/A
HEK-293T Cells ATCC, Manassas, VA CRL-3216
RPMI 1640, 1X with L-glutamine Corning™, Corning, NY 10-040-CV
10% Fetal Bovine Serum Corning™, Corning, NY 35-015-CV
Penicillin-Streptomycin Solution, 100X Corning™, Corning, NY 30-002-CI
G-418 Sulfate Corning™, Corning, NY 30-234-CI
Immunocult™ CD3/28 T Cell Activator StemCell Technologies™, Vancouver, Canada 10991
Human IL-2 IS, premium grade MACS Miltenyi Biotec, Bergisch Gladbach, Germany 130-097-746
All indicated peptides Synthesized by Pierce Thermo Fisher N/A
DMEM, 1X with L-Glutamine, 4.5g/L Glucose and Sodium Pyruvate Corning™, Corning, NY 10-013-CV
Clontech In-Fusion® HD Cloning Kit Clontech, Takara Bio USA, Mountain View, CA 639650
BsmBI New England BioLabs®, Inc, Ipswich, MA R0580S
Clontech Stellar™ Competent Cells Clontech, Takara Bio USA, Mountain View, CA 636763
Zyppy™ Plasmid Miniprep Kit Zymo Research, Irvine, CA D4036
Carbenicillin (disodium) Gold Biotechnology®, Saint Louis, MO C-103-SL10
NucleoBond® Xtra Maxi EF Kit Clontech, Takara Bio USA, Mountain View, CA 740424.50
TransIT®-293 Transfection Reagent Mirus® Bio, LLC Madison, WI MIR 2704
RetroNectin Takara Bio Inc, CA T100B
Gibco™ Opti-MEM™ I Reduced Serum Medium Life Technologies, Thermo Fisher Scientific, Waltham, MA 31985-062
Millex®-HV Syringe Filter Unit, 0.45 µm, PVDF, 33 mm, gamma sterilized EMD Millipore, Burlington, MA SLHV033RS
MACSQuant® Analyzer 10 MACS Miltenyi Biotec, Bergisch Gladbach, Germany 130-096-343
APC/Cy7 anti-human CD69 Clone: FN50 BioLegend®, San Diego, CA 310913
BD FACSort™ Becton Dickinson, Franklin Lakes, NJ
CFSE Cell Division Tracker Kit BioLegend®, San Diego, CA 423801
Invitrogen™ PureLink™ Genomic DNA Mini Kit Invitrogen™ Life Technologies, Thermo Fisher Scientific, Waltham, MA K182001
KOD DNA Polymerase EMD Millipore, Burlington, MA 71085
Macherey-Nagel NucleoSpin® Gel and PCR Purification Kit Clontech, Takara Bio USA, Mountain View, CA 740609.250
TreeStar FlowJo® Flow Cytometric Data Analysis Software v10 FlowJo, LLC Ashland, Oregon N/A
GraphPad Prism v7 GraphPad, San Diego, California N/A
The plasmids for HLA-A0201-SABR backbone (pCCLc-MND-A0201-SABR-Backbone, ID 119050), HLA-B2705-SABR backbone (pCCLc-MND-B2705-SABR-Backbone, ID 119051), A2-Mart1-SABR (pCCLc-MND-A0201-Mart1-SABR, ID 119052), and B27-KK10-SABR (pCCLc-MND-B2705-KK10-SABR, ID 119053) are available through Addgene Inc.