A simple bead-based method for generating cost-effective co-barcoded sequence reads
Advances in genome sequencing continue to decrease cost and improve quality. However, the diploid information found in most genomes is still lost by the most common sequencing methods. There are several methods currently available for obtaining this haplotype information. However, most of these methods are either time consuming, require expensive equipment and reagents, or both. Here we describe single tube long fragment read (stLFR), a simple barcoded bead-based process capable of near perfect whole genome variant calling and haplotyping. Our method uses equipment available in nearly all molecular biology laboratories and costs approximately 30 dollars a sample. In addition, the data generated from this process is capable of detecting and phasing structural variations and scaffolding contigs. We expect in the future that this process will enable affordable diploid de novo assembly.
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This is a list of supplementary files associated with this preprint. Click to download.
Supplementary Figure 1 Barcode sequence assembly Three ligations are necessary to generate ~3.6 billion different barcodes. The expected sequence at each step of the barcode assembly is displayed.
Supplementary Materials Supplementary Materials
Posted 11 Oct, 2018
A simple bead-based method for generating cost-effective co-barcoded sequence reads
Posted 11 Oct, 2018
Advances in genome sequencing continue to decrease cost and improve quality. However, the diploid information found in most genomes is still lost by the most common sequencing methods. There are several methods currently available for obtaining this haplotype information. However, most of these methods are either time consuming, require expensive equipment and reagents, or both. Here we describe single tube long fragment read (stLFR), a simple barcoded bead-based process capable of near perfect whole genome variant calling and haplotyping. Our method uses equipment available in nearly all molecular biology laboratories and costs approximately 30 dollars a sample. In addition, the data generated from this process is capable of detecting and phasing structural variations and scaffolding contigs. We expect in the future that this process will enable affordable diploid de novo assembly.
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