This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
NATIVE: Nanobody-assisted tissue immunostaining for volumetric EM
Hidde Ploegh
Boston Children's Hospital
Corresponding Author
Jeff Lichtman
Harvard University
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
Keywords
CLEM, nanobody, ultrastructure, immunostaining, connectomics
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Oct, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
NATIVE: Nanobody-assisted tissue immunostaining for volumetric EM
Hidde Ploegh
Boston Children's Hospital
Corresponding Author
Jeff Lichtman
Harvard University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 17 Oct, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Morphological and molecular characteristics determine the function of biological tissues. Attempts to combine immunofluorescence and electron microscopy invariably compromise the quality of the ultrastructure of tissue sections. We developed NATIVE, a correlated light and electron microscopy approach that preserves ultrastructure while showing the locations of multiple molecular moieties even deep within tissues. This technique allowed the large-scale 3D reconstruction of a volume of mouse hippocampal CA3 tissue at nanometer resolution.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5