An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jeremy A. Barry
Bioimaging (GlaxoSmithKline) -- Bioimaging, Platform Technology & Science, GlaxoSmithKline, Collegeville, Pennsylvania, 19426, USA
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A revised methodology is presented for the preparation of a mimetic tissue model for use in quantitative imaging mass spectrometry (IMS). Tissue homogenates spiked with known concentrations of a compound standard are serially added to a mold and are snap frozen. The resulting mimetic tissue plug is then cryosectioned and thaw-mounted on the same slide as the tissue(s) to be imaged and quantified. From here, the sample tissue(s) and mimetic tissue model undergo identical sample preparation conditions for IMS. Once IMS is performed, the known analyte concentrations can be correlated with the average intensity of each layer of the mimetic model to generate a calibration curve. This standard curve can then be used to estimate the quantity of analyte that is present in the tissue(s) of interest. The matrix-matched standards applied in this approach account for common IMS issues such as ion suppression and extraction efficiency.
Keywords
Imaging Mass Spectrometry, Quantification, Mimetic Tissue Model
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
The authors declare no competing financial interests.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.xlsm
Supplementary Document 1 Mimetic Tissue Model Calculations.xlsm This macro-enabled spreadsheet can aid in the calculations involved with the construction of the Mimetic Tissue Model
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 24 Aug, 2018
Community comments: 4
Metrics
Comments: 0
HTML Views: ...
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Jeremy A. Barry
Bioimaging (GlaxoSmithKline) -- Bioimaging, Platform Technology & Science, GlaxoSmithKline, Collegeville, Pennsylvania, 19426, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 24 Aug, 2018
Community comments: 4
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A revised methodology is presented for the preparation of a mimetic tissue model for use in quantitative imaging mass spectrometry (IMS). Tissue homogenates spiked with known concentrations of a compound standard are serially added to a mold and are snap frozen. The resulting mimetic tissue plug is then cryosectioned and thaw-mounted on the same slide as the tissue(s) to be imaged and quantified. From here, the sample tissue(s) and mimetic tissue model undergo identical sample preparation conditions for IMS. Once IMS is performed, the known analyte concentrations can be correlated with the average intensity of each layer of the mimetic model to generate a calibration curve. This standard curve can then be used to estimate the quantity of analyte that is present in the tissue(s) of interest. The matrix-matched standards applied in this approach account for common IMS issues such as ion suppression and extraction efficiency.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
The authors declare no competing financial interests.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.xlsm
Supplementary Document 1 Mimetic Tissue Model Calculations.xlsm This macro-enabled spreadsheet can aid in the calculations involved with the construction of the Mimetic Tissue Model
Loading...