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Method Article
Revised Preparation of a Mimetic Tissue Model for Quantitative Imaging Mass Spectrometry
Jeremy A. Barry
Bioimaging (GlaxoSmithKline) -- Bioimaging, Platform Technology & Science, GlaxoSmithKline, Collegeville, Pennsylvania, 19426, USA
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A revised methodology is presented for the preparation of a mimetic tissue model for use in quantitative imaging mass spectrometry (IMS). Tissue homogenates spiked with known concentrations of a compound standard are serially added to a mold and are snap frozen. The resulting mimetic tissue plug is then cryosectioned and thaw-mounted on the same slide as the tissue(s) to be imaged and quantified. From here, the sample tissue(s) and mimetic tissue model undergo identical sample preparation conditions for IMS. Once IMS is performed, the known analyte concentrations can be correlated with the average intensity of each layer of the mimetic model to generate a calibration curve. This standard curve can then be used to estimate the quantity of analyte that is present in the tissue(s) of interest. The matrix-matched standards applied in this approach account for common IMS issues such as ion suppression and extraction efficiency.
Keywords
Imaging Mass Spectrometry, Quantification, Mimetic Tissue Model
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This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.xlsm
Supplementary Document 1 Mimetic Tissue Model Calculations.xlsm This macro-enabled spreadsheet can aid in the calculations involved with the construction of the Mimetic Tissue Model
Chunyan LAN
commented on 28 April, 2019
This is a very good papers.Can you send full text by email?
View 1 reply
Rob
commented on 02 May, 2019
Dear Jeremy, ths is a nice approach. Can you send me the full paper? Warm regards Rob Vreeken
View 1 reply
Jeremy Barry
ORCiDreplied on 06 May, 2019
Hello Rob, Please send me an email at [email protected] and I would be happy to reply with a pdf version of the protocol. Regards, Jeremy Barry
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This is a preprint. It has not completed peer review.
Method Article
Revised Preparation of a Mimetic Tissue Model for Quantitative Imaging Mass Spectrometry
Jeremy A. Barry
Bioimaging (GlaxoSmithKline) -- Bioimaging, Platform Technology & Science, GlaxoSmithKline, Collegeville, Pennsylvania, 19426, USA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 24 Aug, 2018
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
A revised methodology is presented for the preparation of a mimetic tissue model for use in quantitative imaging mass spectrometry (IMS). Tissue homogenates spiked with known concentrations of a compound standard are serially added to a mold and are snap frozen. The resulting mimetic tissue plug is then cryosectioned and thaw-mounted on the same slide as the tissue(s) to be imaged and quantified. From here, the sample tissue(s) and mimetic tissue model undergo identical sample preparation conditions for IMS. Once IMS is performed, the known analyte concentrations can be correlated with the average intensity of each layer of the mimetic model to generate a calibration curve. This standard curve can then be used to estimate the quantity of analyte that is present in the tissue(s) of interest. The matrix-matched standards applied in this approach account for common IMS issues such as ion suppression and extraction efficiency.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Chunyan LAN
commented on 28 April, 2019
This is a very good papers.Can you send full text by email?
View 1 reply
Jeremy Barry
replied on 30 April, 2019
Hi Chunyan, Feel free to send me an email ([email protected]) and I will send over a copy.
Rob
commented on 02 May, 2019
Dear Jeremy, ths is a nice approach. Can you send me the full paper? Warm regards Rob Vreeken
View 1 reply
Jeremy Barry
ORCiDreplied on 06 May, 2019
Hello Rob, Please send me an email at [email protected] and I would be happy to reply with a pdf version of the protocol. Regards, Jeremy Barry
Jeremy Barry
replied on 30 April, 2019
Hi Chunyan, Feel free to send me an email ([email protected]) and I will send over a copy.