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Method Article
Using NEPC cell NCI-H660 for in vitro assays
Xinpei Ci
Vancouver Prostate Centre Department of Urologic Sciences, University of British Columbia, 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada
Corresponding Author
Yuzhuo Wang
Vancouver Prostate Centre Department of Urologic Sciences, University of British Columbia, 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Neuroendocrine prostate cancer (NEPC) is a lethal subtype of prostate cancer (PCa). Research of NEPC has been hampered by a lack of clinically relevant in vitro cell models. NCI-H660 cell is the unique bona fide NEPC cell line derived from a patient, available for in vitro study of NEPC disease. However, this cell grows slowly as floating attached clusters, making regular in vitro cell assays such as proliferation assay and colony formation assay challenging. This protocol provides a simple and practical solution to this technical hurdle.
Keywords
NEPC, NCI-H660
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Associated Publications
Heterochromatin Protein 1α Mediates Development and Aggressiveness of Neuroendocrine Prostate Cancer
by Xinpei Ci, Jun Hao, Xin Dong, +16
Cancer Research (26 July, 2018)
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This is a preprint. It has not completed peer review.
Method Article
Using NEPC cell NCI-H660 for in vitro assays
Xinpei Ci
Vancouver Prostate Centre Department of Urologic Sciences, University of British Columbia, 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada
Corresponding Author
Yuzhuo Wang
Vancouver Prostate Centre Department of Urologic Sciences, University of British Columbia, 2660 Oak Street, Vancouver, BC V6H 3Z6, Canada
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 20 Aug, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Neuroendocrine prostate cancer (NEPC) is a lethal subtype of prostate cancer (PCa). Research of NEPC has been hampered by a lack of clinically relevant in vitro cell models. NCI-H660 cell is the unique bona fide NEPC cell line derived from a patient, available for in vitro study of NEPC disease. However, this cell grows slowly as floating attached clusters, making regular in vitro cell assays such as proliferation assay and colony formation assay challenging. This protocol provides a simple and practical solution to this technical hurdle.
Associated Publications
Heterochromatin Protein 1α Mediates Development and Aggressiveness of Neuroendocrine Prostate Cancer
by Xinpei Ci, Jun Hao, Xin Dong, +16
Cancer Research (26 July, 2018)