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Method Article
Erik J. Sontheimer
University of Massachusetts Medical School
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear and chromosome biology.
Keywords
Cas9, APEX2, Biotinylation, Subnuclear, Telomere, Centromere
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There are no conflicts of financial interest associated with this project.
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Method Article
Erik J. Sontheimer
University of Massachusetts Medical School
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 May, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Mapping proteomic composition at distinct genomic loci and subnuclear landmarks in living cells has been a long-standing challenge. Here we report that dCas9-APEX2 Biotinylation at genomic Elements by Restricted Spatial Tagging (C-BERST) allows the unbiased mapping of proteomes near defined genomic loci, as demonstrated for telomeres and centromeres. C-BERST enables the high-throughput identification of proteins associated with specific sequences, facilitating annotation of these factors and their roles in nuclear and chromosome biology.
Figure 1
Figure 2
Figure 3
Figure 4
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