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Method Article
Paired-end analysis of transcriptional start sites
Ting Ni
Duke University
Corresponding Author
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We describe an improved deep sequencing strategy, paired-end analysis of transcriptional start sites (PEAT), to globally characterize transcriptional start sites (TSSs) in a previous study [1]. It was used to explore the landscape of transcription initiation in Drosophila melanogaster embryo. Here we present a detailed protocol, with minor modifications, can be broadly applied to investigate capped transcripts in other eukaryotes, from budding yeast to rat and human cell lines.
Keywords
Transcriptional start sites, Paired-end sequencing, Initiation motif, Internally capped transcripts
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Associated Publications
A paired-end sequencing strategy to map the complex landscape of transcription initiation
by Ting Ni, David L Corcoran, Elizabeth A Rach, +5
Nature Methods (22 May, 2012)
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This is a preprint. It has not completed peer review.
Method Article
Paired-end analysis of transcriptional start sites
Ting Ni
Duke University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 21 Jul, 2010
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
We describe an improved deep sequencing strategy, paired-end analysis of transcriptional start sites (PEAT), to globally characterize transcriptional start sites (TSSs) in a previous study [1]. It was used to explore the landscape of transcription initiation in Drosophila melanogaster embryo. Here we present a detailed protocol, with minor modifications, can be broadly applied to investigate capped transcripts in other eukaryotes, from budding yeast to rat and human cell lines.
Figure 1
Figure 2
Figure 3
Associated Publications
A paired-end sequencing strategy to map the complex landscape of transcription initiation
by Ting Ni, David L Corcoran, Elizabeth A Rach, +5
Nature Methods (22 May, 2012)