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Method Article
Bacterial proteomic workflow
Hanna Sidjabat
Hanna Sidjabat Group
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol desribes the bacterial proteomic workflow used in the article: "The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapenemase-producing Escherichia coli under antibiotic pressure" published in Scientific Reports.
The workflow begins with growing the bacterial strains, isolating the bacterial proteins, processing the samples for submission to LCMS (protein quantitation, digestion, clean up, desalting). Then, once the samples are being processed on LCMS, the data analysis requires high performance computers.
Keywords
Proteome, bacteria, protein expression, probiotics, pathogens
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This is a preprint. It has not completed peer review.
Method Article
Bacterial proteomic workflow
Hanna Sidjabat
Hanna Sidjabat Group
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Jun, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol desribes the bacterial proteomic workflow used in the article: "The use of SWATH to analyse the dynamic changes of bacterial proteome of carbapenemase-producing Escherichia coli under antibiotic pressure" published in Scientific Reports.
The workflow begins with growing the bacterial strains, isolating the bacterial proteins, processing the samples for submission to LCMS (protein quantitation, digestion, clean up, desalting). Then, once the samples are being processed on LCMS, the data analysis requires high performance computers.