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Method Article
Nicolas Rivron
Nicolas Rivron's Lab
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The blastocyst is the mammalian pre-implantation embryo. It consists of an outer epithelial layer of trophoblast cells surrounding a fluid-filled cavity sheltering the embryonic and primitive endoderm cells. From the mouse blastocyst, trophoblast and embryonic stem cells lines can be derived, which have the capacity to indefinitely self-renew in vitro while maintaining the ability to differentiate into all placental and embryonic lineages, respectively. Here, I describe how to form blastoids (synthetic blastocysts) formed from trophoblast and embryonic stem cells. Blastoids morphologically and transcriptionally resemble E3.5 blastocysts, and can implant in utero. Although not recapitulating the full complexity of the embryo, blastoids are simplified models allowing new approaches in embryology. Contrary to blastocysts, blastoids can be generated in large numbers, which allows to harvest material for in-depth assays (e.g. genomics, biochemistry), run high-throughput screens, and to rapidly perform compartment-specific genetic modifications. As such, blastoids are powerful tools to study the principles of stem cell self-organization and embryonic development.
Note: Minor errors in this protocol were corrected on the 18th of May 2018.
The previous version of the protocol can be found here;
Keywords
Blastoid; trophectoderm; morphogenesis; embryonic inductions; trophoblast stem cells; embryonic stem cells; endometrium; uterus; implantation.
Figure 1
Nicolas Rivron is an inventor on the patent US14/784,659 and PCT/NL2014/050239 (April 2014) describing the blastoid technology.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Complete Protocol - Version 1 In vitro generation of blastoids from trophoblast and embryonic stem cells This is the version of the protocol that was originally published. It was updated on the 12 May 2018 to correct errors in the text.
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Associated Publications
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Nicolas Rivron
Nicolas Rivron's Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 14 May, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The blastocyst is the mammalian pre-implantation embryo. It consists of an outer epithelial layer of trophoblast cells surrounding a fluid-filled cavity sheltering the embryonic and primitive endoderm cells. From the mouse blastocyst, trophoblast and embryonic stem cells lines can be derived, which have the capacity to indefinitely self-renew in vitro while maintaining the ability to differentiate into all placental and embryonic lineages, respectively. Here, I describe how to form blastoids (synthetic blastocysts) formed from trophoblast and embryonic stem cells. Blastoids morphologically and transcriptionally resemble E3.5 blastocysts, and can implant in utero. Although not recapitulating the full complexity of the embryo, blastoids are simplified models allowing new approaches in embryology. Contrary to blastocysts, blastoids can be generated in large numbers, which allows to harvest material for in-depth assays (e.g. genomics, biochemistry), run high-throughput screens, and to rapidly perform compartment-specific genetic modifications. As such, blastoids are powerful tools to study the principles of stem cell self-organization and embryonic development.
Note: Minor errors in this protocol were corrected on the 18th of May 2018.
The previous version of the protocol can be found here;
Figure 1
Nicolas Rivron is an inventor on the patent US14/784,659 and PCT/NL2014/050239 (April 2014) describing the blastoid technology.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Complete Protocol - Version 1 In vitro generation of blastoids from trophoblast and embryonic stem cells This is the version of the protocol that was originally published. It was updated on the 12 May 2018 to correct errors in the text.
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