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Method Article
High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robot
Dominic Grün
MPI-IE
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
For the detection of subtle transcriptome changes reflecting fate biases, an scRNA-seq method is required that maximizes both sensitivity and accuracy. Recent benchmarking has demonstrated that CEL-Seq2 optimized both of these factors, but comes with high costs per cell (Hashimshony, T. et al. Genome Biol. 17, 77, 2016; Svensson, V. et al. Nat. Methods 14, 381–387, 2017). To enable high-throughput sequencing by the CEL-Seq2 method we here establish a robotic version of this method that permits sequencing of thousands of cells at substantially reduced costs.
Keywords
CEL-Seq2, single cell sequencing, nanoliter pipetting, high-throughput, mosquito, mCEL-Seq2, 384-well plate
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- supplement0.txt
192 unique polydT primer sequences 192 unique polydT primer sequences
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This is a preprint. It has not completed peer review.
Method Article
High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robot
Dominic Grün
MPI-IE
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 24 May, 2018
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
For the detection of subtle transcriptome changes reflecting fate biases, an scRNA-seq method is required that maximizes both sensitivity and accuracy. Recent benchmarking has demonstrated that CEL-Seq2 optimized both of these factors, but comes with high costs per cell (Hashimshony, T. et al. Genome Biol. 17, 77, 2016; Svensson, V. et al. Nat. Methods 14, 381–387, 2017). To enable high-throughput sequencing by the CEL-Seq2 method we here establish a robotic version of this method that permits sequencing of thousands of cells at substantially reduced costs.