High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robot
For the detection of subtle transcriptome changes reflecting fate biases, an scRNA-seq method is required that maximizes both sensitivity and accuracy. Recent benchmarking has demonstrated that CEL-Seq2 optimized both of these factors, but comes with high costs per cell (Hashimshony, T. et al. Genome Biol. 17, 77, 2016; Svensson, V. et al. Nat. Methods 14, 381–387, 2017). To enable high-throughput sequencing by the CEL-Seq2 method we here establish a robotic version of this method that permits sequencing of thousands of cells at substantially reduced costs.
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192 unique polydT primer sequences 192 unique polydT primer sequences
Posted 24 May, 2018
High-throughput single cell sequencing using CEL-Seq2 on a nanoliter dispensing robot
Posted 24 May, 2018
For the detection of subtle transcriptome changes reflecting fate biases, an scRNA-seq method is required that maximizes both sensitivity and accuracy. Recent benchmarking has demonstrated that CEL-Seq2 optimized both of these factors, but comes with high costs per cell (Hashimshony, T. et al. Genome Biol. 17, 77, 2016; Svensson, V. et al. Nat. Methods 14, 381–387, 2017). To enable high-throughput sequencing by the CEL-Seq2 method we here establish a robotic version of this method that permits sequencing of thousands of cells at substantially reduced costs.
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