Bead suspension (radius of 2.32 μm, SS04N, Bangs Labs, Fishers, IN)
Millipore water (Billerica, MA)
TOTO-1 iodide nucleic acid stain (ThermoFisher Scientific, Molecular probes, USA)
Fluoresceine sodium salt (ThermoFisher Scientific, USA)
UV curing glue
Non-bleaching mixture of vaseline, paraffine, and lanoline
Silicone paste
Filter paper
Cover slips (60×24 mm2, #1.5 and 20×20 mm2, #1.5)
Glass slides (75×25×1 mm3)
Diagnostic slides (10 well / 6 mm)
Spacers (e.g. cut from a book wrapping foil)
Grids covered with Formvar support film for TEM (SPI supplies, USA)
Metal holders for SEM
1 % (w/v) aqueous uranyl acetate
1 % (v/v) formaldehyde and 0.5 % (v/v) glutaraldehyde in a 0.1 M cacodylate buffer
0.1 M cacodylate buffer (pH 7.4)
1 % (v/v) aqueous solution of osmium tetroxide (SPI supplies, USA)
Acetone (absolute)
1,1,1,3,3,3-Hexamethyldisilazane (Merck, Germany)
1,1,1,3,3,3-Hexamethyldisilazane concentration series in absolute acetone (30, 60, and 100 % (v/v))
Ethanol concentration series (e.g. 50, 70, 90, 96, and 100 % (v/v))
Phosphate-buffered saline solution (PBS): 8.00 g/l NaCl, 0.20 g/l KCl, 1.44 g/l Na2HPO4, and 0.24 g/l KH2PO4, pH 7.4
SlowFade Gold antifade reagent (Life technologies, Termo Fisher Scientific, USA)
Lysogeny broth agar plates (LB plates): 1.0 % (w/v) tryptone, 0.5 % (w/v) yeast extract, 1.0 % (w/v) NaCl, and 1.5 % (w/v) agar
Lysogeny broth liquid medium (LB): 1.0 % (w/v) tryptone, 0.5 % (w/v) yeast extract, and 1.0 % (w/v) NaCl
SYM liquid medium (1): 70 mM K2HPO4, 30 mM KH2PO4, 25 mM (NH4)2SO4, 0.5 mM MgSO4, 0.01 mM MnSO4, 22 mg/l ammonium iron (III) citrate, 2 % (w/v) yeast extract, and 20 % (w/v) sucrose
SM liquid medium (SYM medium without yeast extract)
M9 liquid medium: 6.4 % (w/v) Na2HPO4 × 7H2O, 1.5 % (w/v) KH2PO4, 0.25 % (w/v) NaCl, 0.5 % (w/v) NH4Cl, 2 mM MgSO4, 0.4 % (w/v) glucose, and 0.1 mM CaCl2