In vitro analysis of cytotoxic T cell recruitment mediated by the DC-derived chemokine CCL17

doi:10.1038/nprot.2010.51

This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.

Method Article

Thomas Quast, Verena Semmling, Christian Kurts, Irmgard Förster, Waldemar Kolanus

Thomas Quast

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Verena Semmling

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Christian Kurts

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Irmgard Förster

Molecular Immunology, Institut für Umweltmedizinische Forschung, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany

Waldemar Kolanus

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

DOI:

10.1038/nprot.2010.51

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Figure 1

Figure 2

Version History

CURRENT STATUS: Posted

Version 1

Posted 05 Mar, 2010

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Associated Publications

Alternative cross-priming through CCL17-CCR4-mediated attraction of CTLs toward NKT cell–licensed DCs
by Verena Semmling, Veronika Lukacs-Kornek, Christoph A Thaiss, +11
Nature Immunology (24 February, 2010)

Method Article

Thomas Quast, Verena Semmling, Christian Kurts, Irmgard Förster, Waldemar Kolanus

Thomas Quast

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Verena Semmling

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Christian Kurts

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Irmgard Förster

Molecular Immunology, Institut für Umweltmedizinische Forschung, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany

Waldemar Kolanus

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Version History

CURRENT STATUS: Posted

Version 1

Posted 05 Mar, 2010

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Biological techniques

DOI:

10.1038/nprot.2010.51

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Figure 1

Figure 2

Associated Publications

Alternative cross-priming through CCL17-CCR4-mediated attraction of CTLs toward NKT cell–licensed DCs
by Verena Semmling, Veronika Lukacs-Kornek, Christoph A Thaiss, +11
Nature Immunology (24 February, 2010)

Method Article

Thomas Quast, Verena Semmling, Christian Kurts, Irmgard Förster, Waldemar Kolanus

Thomas Quast

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Verena Semmling

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Christian Kurts

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Irmgard Förster

Molecular Immunology, Institut für Umweltmedizinische Forschung, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany

Waldemar Kolanus

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

DOI:

10.1038/nprot.2010.51

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Figures

Introduction

Reagents

Equipment

Procedure

Timing

Troubleshooting

Anticipated Results

References

Acknowledgements

Comments

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Associated Publications

Alternative cross-priming through CCL17-CCR4-mediated attraction of CTLs toward NKT cell–licensed DCs

by Verena Semmling, Veronika Lukacs-Kornek, Christoph A Thaiss, +11

Nature Immunology (24 February, 2010)

Method Article

Thomas Quast, Verena Semmling, Christian Kurts, Irmgard Förster, Waldemar Kolanus

Thomas Quast

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Verena Semmling

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Christian Kurts

Institutes of Molecular Medicine and Experimental Immunology, Friedrich-Wilhelms-Universität, Bonn, Germany.

Irmgard Förster

Molecular Immunology, Institut für Umweltmedizinische Forschung, Heinrich-Heine-Universität, 40225 Düsseldorf, Germany

Waldemar Kolanus

Life&Medical Sciences (LIMES) Institute, Friedrich-Wilhelms-Universität, 53115 Bonn, Germany

Badges

Peer Review Timeline

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Subject Areas

Biological techniques

DOI & PDF

DOI:

10.1038/nprot.2010.51

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Declarations

Figures

Introduction

Reagents

Equipment

Procedure

Timing