qPCR based reporter assay on luciferase transcripts
This protocol describes a reporter assay that measures the abundance of firefly transcripts by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase transcripts which will be normalized to constitutively expressed Renilla transcripts.
Posted 11 Dec, 2017
qPCR based reporter assay on luciferase transcripts
Posted 11 Dec, 2017
This protocol describes a reporter assay that measures the abundance of firefly transcripts by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase transcripts which will be normalized to constitutively expressed Renilla transcripts.
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