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Method Article
qPCR based reporter assay on luciferase transcripts
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a reporter assay that measures the abundance of firefly transcripts by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase transcripts which will be normalized to constitutively expressed Renilla transcripts.
Keywords
enhancer, reporter assay, luciferase, starr-seq, mpra
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Posted 11 Dec, 2017
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Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534
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This is a preprint. It has not completed peer review.
Method Article
qPCR based reporter assay on luciferase transcripts
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Dec, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a reporter assay that measures the abundance of firefly transcripts by qPCR as a readout for enhancer activity. Similar to a classical luciferase assay, which uses the enzymatic activity of the luciferase, this assay measures abundance of luciferase transcripts which will be normalized to constitutively expressed Renilla transcripts.
Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534