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Method Article
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a qPCR assay that measures the expression of endogenous interferon stimulated genes (ISGs) after the transfection of exogenous DNA into human cells.
Keywords
enhancer, reporter assay, luciferase, starr-seq, mpra
The authors declare no competing financial interests.
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CURRENT STATUS: Posted
Version 1
Posted 11 Dec, 2017
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Subject Areas
Biotechnology
Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 11 Dec, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a qPCR assay that measures the expression of endogenous interferon stimulated genes (ISGs) after the transfection of exogenous DNA into human cells.
The authors declare no competing financial interests.
Loading...
Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534