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Method Article
qPCR assay to measure ISG expression in human cells
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a qPCR assay that measures the expression of endogenous interferon stimulated genes (ISGs) after the transfection of exogenous DNA into human cells.
Keywords
enhancer, reporter assay, luciferase, starr-seq, mpra
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Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534
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This is a preprint. It has not completed peer review.
Method Article
qPCR assay to measure ISG expression in human cells
Alexander Stark
Research Institute of Molecular Pathology (IMP), Vienna Biocenter (VBC), Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 11 Dec, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes a qPCR assay that measures the expression of endogenous interferon stimulated genes (ISGs) after the transfection of exogenous DNA into human cells.
Associated Publications
STARR-seq — Principles and applications
by Felix Muerdter, Łukasz M. Boryń, and Cosmas D. Arnold
Genome-Wide Quantitative Enhancer Activity Maps Identified by STARR-seq
by C. D. Arnold, D. Gerlach, C. Stelzer, +3
Science (13 November, 2017)
10.1038/nmeth.4534