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Method Article
Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition
Gabriel C. Lander
Lander Lab, The Scripps Research Institute
Corresponding Author
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It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.
Keywords
cryo-EM, electron microscopy, 200 kev, Talos Arctica, Gatan K2
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Associated Publications
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition
Gabriel C. Lander
Lander Lab, The Scripps Research Institute
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.
Associated Publications
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)