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Method Article
Gabriel C. Lander
Lander Lab, The Scripps Research Institute
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.
Keywords
cryo-EM, electron microscopy, 200 kev, Talos Arctica, Gatan K2
None
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Associated Publications
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)
Method Article
Gabriel C. Lander
Lander Lab, The Scripps Research Institute
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Oct, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.
Loading...
Associated Publications
Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)