Setting up the Talos Arctica electron microscope and Gatan K2 direct detector for high-resolution cryogenic single-particle data acquisition

doi:10.1038/protex.2017.108

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Method Article

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu

Gabriel C. Lander

Lander Lab, The Scripps Research Institute

Corresponding Author

Mark A. Herzik, Jr.

Mengyu Wu

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It has been determined that a Talos Arctica transmission electron microscope (TEM) is capable of resolving frozen-hydrated macromolecules of various sizes and internal symmetries to better than 3 Å resolution using single particle methodologies. This protocol describes how to maximize parallel illumination conditions in a Talos Arctica TEM operating at 200 keV for high-resolution cryogenic data acquisition for structure determination.

Keywords

cryo-EM, electron microscopy, 200 kev, Talos Arctica, Gatan K2

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Associated Publications

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu

Gabriel C. Lander

Lander Lab, The Scripps Research Institute

Corresponding Author

Mark A. Herzik, Jr.

Mengyu Wu

Version History

CURRENT STATUS: Posted

Version 1

Posted 12 Oct, 2017

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Biological techniques

Structural biology

DOI:

10.1038/protex.2017.108

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY 4.0 License. Read Full License

None

Associated Publications

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV
by Mark A Herzik, Mengyu Wu, and Gabriel C Lander
Nature Methods (12 October, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu

Gabriel C. Lander

Lander Lab, The Scripps Research Institute

Corresponding Author

Mark A. Herzik, Jr.

Mengyu Wu

DOI:

10.1038/protex.2017.108

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

cryo-EM, electron microscopy, 200 kev, Talos Arctica, Gatan K2

Introduction

Reagents

Equipment

Procedure

References

Competing Interests

Comments

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CURRENT STATUS: Posted

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Subject Areas

Biological techniques

Structural biology

Associated Publications

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV

by Mark A Herzik, Mengyu Wu, and Gabriel C Lander

Nature Methods (12 October, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Gabriel C. Lander, Mark A. Herzik, Jr., Mengyu Wu

Gabriel C. Lander

Lander Lab, The Scripps Research Institute

Corresponding Author

Mark A. Herzik, Jr.

Mengyu Wu

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CURRENT STATUS: Posted

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DOI & PDF

DOI:

10.1038/protex.2017.108

Download PDF

License

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure

References

Competing Interests

Comments

Related Articles

Associated Publications

Achieving better-than-3-Å resolution by single-particle cryo-EM at 200 keV