Isolation and functional characterization of human glioblastoma stem cells

doi:10.1038/protex.2017.123

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Method Article

Xiaoyang Lan, Peter B. Dirks, Lilian Lee, Michelle M. Kushida, Clare Che, Naghmeh Rastegar

Xiaoyang Lan

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Peter B. Dirks

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Lilian Lee

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Michelle M. Kushida

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Clare Che

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Naghmeh Rastegar

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

DOI:

10.1038/protex.2017.123

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Here, we describe protocols for the isolation, expansion, and functional characterization of human glioblastoma stem cells.

Keywords

Glioblastoma, Cancer Stem Cells

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The authors declare no competing financial interests.

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Cell biology

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Associated Publications

Fate mapping of human glioblastoma reveals an invariant stem cell hierarchy
by Xiaoyang Lan, David J. Jörg, Florence M. G. Cavalli, +12
Nature (31 August, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Xiaoyang Lan, Peter B. Dirks, Lilian Lee, Michelle M. Kushida, Clare Che, Naghmeh Rastegar

Xiaoyang Lan

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Peter B. Dirks

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Lilian Lee

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Michelle M. Kushida

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Clare Che

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Naghmeh Rastegar

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

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Subject Areas

Cell biology

Biological techniques

DOI:

10.1038/protex.2017.123

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Here, we describe protocols for the isolation, expansion, and functional characterization of human glioblastoma stem cells.

Figure 1

Figure 2

The authors declare no competing financial interests.

Associated Publications

Fate mapping of human glioblastoma reveals an invariant stem cell hierarchy
by Xiaoyang Lan, David J. Jörg, Florence M. G. Cavalli, +12
Nature (31 August, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Xiaoyang Lan, Peter B. Dirks, Lilian Lee, Michelle M. Kushida, Clare Che, Naghmeh Rastegar

Xiaoyang Lan

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Peter B. Dirks

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Lilian Lee

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Michelle M. Kushida

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Clare Che

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Naghmeh Rastegar

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

DOI:

10.1038/protex.2017.123

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

Glioblastoma, Cancer Stem Cells

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Introduction

Reagents

Equipment

Procedure

Timing

Troubleshooting

Anticipated Results

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Acknowledgements

Competing Interests

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Associated Publications

Fate mapping of human glioblastoma reveals an invariant stem cell hierarchy

by Xiaoyang Lan, David J. Jörg, Florence M. G. Cavalli, +12

Nature (31 August, 2017)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Xiaoyang Lan, Peter B. Dirks, Lilian Lee, Michelle M. Kushida, Clare Che, Naghmeh Rastegar

Xiaoyang Lan

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Peter B. Dirks

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Corresponding Author

Lilian Lee

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Michelle M. Kushida

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Clare Che

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

Naghmeh Rastegar

Developmental and Stem Cell Biology Program and The Arthur and Sonia Labatt Brain Tumour Research Centre, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.

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