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Method Article
Protocol for Convolutional Neural Networks based Automated Cellular Cryo-Electron Tomograms Annotation
Steven J. Ludtke
Ludtke Lab, Baylor College of Medicine
Corresponding Author
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Cellular Electron Cryotomography (CryoET) offers the ability to look inside cells and observe macromolecules frozen in action. A primary challenge for this technique is identifying and extracting the molecular components within the crowded cellular environment. We introduce a method using convolutional neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction.
Keywords
Cellular Electron Cryotomography, convolutional neural network, tomogram annotation
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Associated Publications
Convolutional neural networks for automated annotation of cellular cryo-electron tomograms
by Muyuan Chen, Wei Dai, Stella Y Sun, +5
Nature Methods (29 August, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Protocol for Convolutional Neural Networks based Automated Cellular Cryo-Electron Tomograms Annotation
Steven J. Ludtke
Ludtke Lab, Baylor College of Medicine
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 29 Aug, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Cellular Electron Cryotomography (CryoET) offers the ability to look inside cells and observe macromolecules frozen in action. A primary challenge for this technique is identifying and extracting the molecular components within the crowded cellular environment. We introduce a method using convolutional neural networks to dramatically reduce the time and human effort required for subcellular annotation and feature extraction.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Associated Publications
Convolutional neural networks for automated annotation of cellular cryo-electron tomograms
by Muyuan Chen, Wei Dai, Stella Y Sun, +5
Nature Methods (29 August, 2017)