This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Bernhard Spengler
Spengler Lab (Justus Liebig University, Giessen)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Mass spectrometry imaging (MSI) is a profiling technique that allows to visualize molecular information from various sample surfaces. In this protocol, we provide the experimental details for surface profiling of three-dimensional (3D) objects, i.e. rough surfaces using LDI or MALDI MSI at ambient pressure. This procedure is applicable to various objects, such as inorganic surfaces, intact plants, insects and imperfect tissue sections. We provide a protocol for matrix application, data analysis and data visualization for the use with a high-resolution atmospheric pressure MALDI MSI source. This protocol is intended for 3D sample imaging with down to 10 µm lateral resolution and about 1.5 µm height resolution. As an example, various substance classes on plant, insect and coin surfaces were imaged. The data analysis, including MS image generation, generation of topographic images and the combination to a multi-dimensional data visualization are described and discussed in this protocol. Compound assignment is based on accurate mass measurements (mass error less than 2 ppm) using a high mass resolution (R=140.000 at m/z=200) orbital trapping mass spectrometer.
Keywords
Mass Spectrometry Imaging, 3D Surface Analysis
Figure 1
Figure 2
Figure 3
Figure 4
B.S. is a consultant of TransMIT GmbH. M.K. is an employee of TransMIT GmbH. S.H. declares no competing financial interests.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
by Mario Kompauer, Sven Heiles, and Bernhard Spengler
Nature Methods (18 September, 2017)
Method Article
Bernhard Spengler
Spengler Lab (Justus Liebig University, Giessen)
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Mass spectrometry imaging (MSI) is a profiling technique that allows to visualize molecular information from various sample surfaces. In this protocol, we provide the experimental details for surface profiling of three-dimensional (3D) objects, i.e. rough surfaces using LDI or MALDI MSI at ambient pressure. This procedure is applicable to various objects, such as inorganic surfaces, intact plants, insects and imperfect tissue sections. We provide a protocol for matrix application, data analysis and data visualization for the use with a high-resolution atmospheric pressure MALDI MSI source. This protocol is intended for 3D sample imaging with down to 10 µm lateral resolution and about 1.5 µm height resolution. As an example, various substance classes on plant, insect and coin surfaces were imaged. The data analysis, including MS image generation, generation of topographic images and the combination to a multi-dimensional data visualization are described and discussed in this protocol. Compound assignment is based on accurate mass measurements (mass error less than 2 ppm) using a high mass resolution (R=140.000 at m/z=200) orbital trapping mass spectrometer.
Figure 1
Figure 2
Figure 3
Figure 4
Loading...
Associated Publications
Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
by Mario Kompauer, Sven Heiles, and Bernhard Spengler
Nature Methods (18 September, 2017)