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Method Article
Chemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging
Bernhard Spengler
Spengler Lab (Justus Liebig University, Giessen)
Corresponding Author
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Mass spectrometry imaging (MSI) is a profiling technique that allows to visualize molecular information from various sample surfaces. In this protocol, we provide the experimental details for surface profiling of three-dimensional (3D) objects, i.e. rough surfaces using LDI or MALDI MSI at ambient pressure. This procedure is applicable to various objects, such as inorganic surfaces, intact plants, insects and imperfect tissue sections. We provide a protocol for matrix application, data analysis and data visualization for the use with a high-resolution atmospheric pressure MALDI MSI source. This protocol is intended for 3D sample imaging with down to 10 µm lateral resolution and about 1.5 µm height resolution. As an example, various substance classes on plant, insect and coin surfaces were imaged. The data analysis, including MS image generation, generation of topographic images and the combination to a multi-dimensional data visualization are described and discussed in this protocol. Compound assignment is based on accurate mass measurements (mass error less than 2 ppm) using a high mass resolution (R=140.000 at m/z=200) orbital trapping mass spectrometer.
Keywords
Mass Spectrometry Imaging, 3D Surface Analysis
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Associated Publications
Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
by Mario Kompauer, Sven Heiles, and Bernhard Spengler
Nature Methods (18 September, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Chemical and topographical 3D surface profiling using atmospheric pressure LDI and MALDI MS imaging
Bernhard Spengler
Spengler Lab (Justus Liebig University, Giessen)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 18 Sep, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Mass spectrometry imaging (MSI) is a profiling technique that allows to visualize molecular information from various sample surfaces. In this protocol, we provide the experimental details for surface profiling of three-dimensional (3D) objects, i.e. rough surfaces using LDI or MALDI MSI at ambient pressure. This procedure is applicable to various objects, such as inorganic surfaces, intact plants, insects and imperfect tissue sections. We provide a protocol for matrix application, data analysis and data visualization for the use with a high-resolution atmospheric pressure MALDI MSI source. This protocol is intended for 3D sample imaging with down to 10 µm lateral resolution and about 1.5 µm height resolution. As an example, various substance classes on plant, insect and coin surfaces were imaged. The data analysis, including MS image generation, generation of topographic images and the combination to a multi-dimensional data visualization are described and discussed in this protocol. Compound assignment is based on accurate mass measurements (mass error less than 2 ppm) using a high mass resolution (R=140.000 at m/z=200) orbital trapping mass spectrometer.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
by Mario Kompauer, Sven Heiles, and Bernhard Spengler
Nature Methods (18 September, 2017)