Drosophila melanogaster is a widely used model organism in many biological fields, due to its short life cycle, highly tractable genome, ease of handling, and many other features. Due to its widespread use, there is a great demand for more effective and efficient methods utilizing Drosophila. We have developed a protocol for homogenization of Drosophila whereby 24 samples of up to 300mg of larvae can be easily and inexpensively homogenized using the Bullet BlenderTM in under 5 minutes for extraction of proteins, nucleic acids, or other analytes. Importantly, the unique technology utilized enables samples to stay cool during homogenization.