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Method Article
Protocol for rapidly inducible Cas9 and DSB-ddPCR
Dustin J. Maly
Maly Lab, University of Washington - Department of Chemistry
Corresponding Author
Douglas M. Fowler
Fowler Lab, University of Washington - Department of Genome Sciences
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
DSB-ddPCR is a droplet digital PCR-based assay for double strand breaks. DSB-ddPCR measures DSBs in a time-resolved, highly quantitative and targeted manner.
Keywords
Double stranded break, digital droplet polymerase chain reaction
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Associated Publications
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
by John C Rose, Jason J Stephany, William J Valente, +5
Nature Methods (12 September, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Protocol for rapidly inducible Cas9 and DSB-ddPCR
Dustin J. Maly
Maly Lab, University of Washington - Department of Chemistry
Corresponding Author
Douglas M. Fowler
Fowler Lab, University of Washington - Department of Genome Sciences
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 12 Sep, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
DSB-ddPCR is a droplet digital PCR-based assay for double strand breaks. DSB-ddPCR measures DSBs in a time-resolved, highly quantitative and targeted manner.
Figure 1
Figure 2
Figure 3
Associated Publications
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics
by John C Rose, Jason J Stephany, William J Valente, +5
Nature Methods (12 September, 2017)