This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Nils Kronenberg
GatherLab (University of St Andrews)
Corresponding Author
Malte C. Gather
GatherLab (University of St Andrews)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes the fabrication of micro-cavity chips for the measurement of cell forces via Elastic Resonator Stress Microscopy as described in the Nature Cell Biology paper “Long-Term Imaging of Cellular Forces with High Precision by Elastic Resonator Interference Stress Microscopy”. We have designed elastic, optical micro-cavities that are built on glass coverslips and consist of a layered structure of an ultra-soft elastomer layer sandwiched between two thin and flexible gold mirrors. Optical readout of the local thickness of micro-cavity chips allows for the detection of minute vertical displacement caused by cells cultured on top of the chip. All steps of the fabrication are carried out under cleanroom conditions
Keywords
Biophysics, cellular biomechanics, cell forces, ERISM, podosome, amoeboid migration, mesenchymal migration, traction force microscopy
The University of St Andrews has filed a patent application related to the protocol described here.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 20 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy
by Nils M. Kronenberg, Philipp Liehm, Anja Steude, +6
Nature Cell Biology (20 June, 2017)
Method Article
Nils Kronenberg
GatherLab (University of St Andrews)
Corresponding Author
Malte C. Gather
GatherLab (University of St Andrews)
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 20 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol describes the fabrication of micro-cavity chips for the measurement of cell forces via Elastic Resonator Stress Microscopy as described in the Nature Cell Biology paper “Long-Term Imaging of Cellular Forces with High Precision by Elastic Resonator Interference Stress Microscopy”. We have designed elastic, optical micro-cavities that are built on glass coverslips and consist of a layered structure of an ultra-soft elastomer layer sandwiched between two thin and flexible gold mirrors. Optical readout of the local thickness of micro-cavity chips allows for the detection of minute vertical displacement caused by cells cultured on top of the chip. All steps of the fabrication are carried out under cleanroom conditions
Loading...
Associated Publications
Long-term imaging of cellular forces with high precision by elastic resonator interference stress microscopy
by Nils M. Kronenberg, Philipp Liehm, Anja Steude, +6
Nature Cell Biology (20 June, 2017)