Fabrication of elastic micro-cavity chips for use in Elastic Resonator Interference Stress Microscopy
This protocol describes the fabrication of micro-cavity chips for the measurement of cell forces via Elastic Resonator Stress Microscopy as described in the Nature Cell Biology paper “Long-Term Imaging of Cellular Forces with High Precision by Elastic Resonator Interference Stress Microscopy”. We have designed elastic, optical micro-cavities that are built on glass coverslips and consist of a layered structure of an ultra-soft elastomer layer sandwiched between two thin and flexible gold mirrors. Optical readout of the local thickness of micro-cavity chips allows for the detection of minute vertical displacement caused by cells cultured on top of the chip. All steps of the fabrication are carried out under cleanroom conditions
Posted 20 Jun, 2017
Fabrication of elastic micro-cavity chips for use in Elastic Resonator Interference Stress Microscopy
Posted 20 Jun, 2017
This protocol describes the fabrication of micro-cavity chips for the measurement of cell forces via Elastic Resonator Stress Microscopy as described in the Nature Cell Biology paper “Long-Term Imaging of Cellular Forces with High Precision by Elastic Resonator Interference Stress Microscopy”. We have designed elastic, optical micro-cavities that are built on glass coverslips and consist of a layered structure of an ultra-soft elastomer layer sandwiched between two thin and flexible gold mirrors. Optical readout of the local thickness of micro-cavity chips allows for the detection of minute vertical displacement caused by cells cultured on top of the chip. All steps of the fabrication are carried out under cleanroom conditions
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