An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Joseph R. Ecker
Ecker Lab
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Knowledge from protein interactome mapping can greatly enhance how we interpret genomic and transcriptomic data. However interactome datasets are currently incomplete, limited by the throughput of existing technologies. Here we describe a massively-multiplexed yeast two-hybrid method, CrY2H-seq, for deep coverage interactome mapping. This protocol accompanies Trigg et al (Nature Methods 2017); it was added to the manuscript after formal peer review, as an aid to users.
Keywords
yeast two-hybrid, protein-protein interaction, interactome, sequencing
Figure 1
Figure 2
Figure 3
Figure 4
The authors declare no competing financial interests.
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
Associated Publications
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
by Shelly A Trigg, Renee M Garza, Andrew MacWilliams, +10
Nature Methods (28 June, 2017)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Joseph R. Ecker
Ecker Lab
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 28 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Knowledge from protein interactome mapping can greatly enhance how we interpret genomic and transcriptomic data. However interactome datasets are currently incomplete, limited by the throughput of existing technologies. Here we describe a massively-multiplexed yeast two-hybrid method, CrY2H-seq, for deep coverage interactome mapping. This protocol accompanies Trigg et al (Nature Methods 2017); it was added to the manuscript after formal peer review, as an aid to users.
Figure 1
Figure 2
Figure 3
Figure 4
The authors declare no competing financial interests.
Loading...
Associated Publications
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
by Shelly A Trigg, Renee M Garza, Andrew MacWilliams, +10
Nature Methods (28 June, 2017)