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Method Article
CrY2H-seq interactome screening
Joseph R. Ecker
Ecker Lab
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Knowledge from protein interactome mapping can greatly enhance how we interpret genomic and transcriptomic data. However interactome datasets are currently incomplete, limited by the throughput of existing technologies. Here we describe a massively-multiplexed yeast two-hybrid method, CrY2H-seq, for deep coverage interactome mapping. This protocol accompanies Trigg et al (Nature Methods 2017); it was added to the manuscript after formal peer review, as an aid to users.
Keywords
yeast two-hybrid, protein-protein interaction, interactome, sequencing
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Associated Publications
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
by Shelly A Trigg, Renee M Garza, Andrew MacWilliams, +10
Nature Methods (28 June, 2017)
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This is a preprint. It has not completed peer review.
Method Article
CrY2H-seq interactome screening
Joseph R. Ecker
Ecker Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 28 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Knowledge from protein interactome mapping can greatly enhance how we interpret genomic and transcriptomic data. However interactome datasets are currently incomplete, limited by the throughput of existing technologies. Here we describe a massively-multiplexed yeast two-hybrid method, CrY2H-seq, for deep coverage interactome mapping. This protocol accompanies Trigg et al (Nature Methods 2017); it was added to the manuscript after formal peer review, as an aid to users.
Figure 1
Figure 2
Figure 3
Figure 4
Associated Publications
CrY2H-seq: a massively multiplexed assay for deep-coverage interactome mapping
by Shelly A Trigg, Renee M Garza, Andrew MacWilliams, +10
Nature Methods (28 June, 2017)