Method Article
Circularization for In vitro Reporting of Cleavage Effects (CIRCLE-seq)
https://doi.org/10.1038/protex.2017.147
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posted 08 Nov, 2017
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CIRCLE-seq is a sensitive method for selective sequencing of Cas9-cleaved genomic DNA
molecules. Briefly, genomic DNA is circularized using a restriction-enzyme independent approach.
Successfully circularized molecules are enzymatically selected for by exonuclease treatment and
then treated with Cas9-gRNA complex. Only Cas9-linearized molecules can be subsequently
ligated to adapters for high-throughput sequencing.
CIRCLE-seq
Refer to the "attached file with the full procedure":https://www.nature.com/protocolexchange/system/uploads/5611/original/CIRCLE-seq_Supplementary_Protocol.pdf?1493740820
J.K.J. is a consultant for Horizon Discovery. J.K.J. has financial interests in Beacon Genomics, Editas Medicine, Poseida Therapeutics, and Transposagen Biopharmaceuticals. J.K.J.’s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies. S.Q.T., M.J.A., and J.K.J. are scientific cofounders of Beacon Genomics.
Supplementary document 1: CIRCLE-seq protocol CIRCLE-seq protocol
This protocol has been posted on Protocol Exchange, an open repository of community-contributed protocols sponsored by Nature Portfolio. These protocols are posted directly on the Protocol Exchange by authors and are made freely available to the scientific community for use and comment.
posted 08 Nov, 2017
You are reading this latest protocol version