Circularization for In vitro Reporting of Cleavage Effects (CIRCLE-seq)
CIRCLE-seq is a sensitive method for selective sequencing of Cas9-cleaved genomic DNA
molecules. Briefly, genomic DNA is circularized using a restriction-enzyme independent approach.
Successfully circularized molecules are enzymatically selected for by exonuclease treatment and
then treated with Cas9-gRNA complex. Only Cas9-linearized molecules can be subsequently
ligated to adapters for high-throughput sequencing.
Refer to the "attached file with the full procedure":https://www.nature.com/protocolexchange/system/uploads/5611/original/CIRCLE-seq_Supplementary_Protocol.pdf?1493740820
This is a list of supplementary files associated with this preprint. Click to download.
Supplementary document 1: CIRCLE-seq protocol CIRCLE-seq protocol
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Posted 08 Nov, 2017
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