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Method Article
Shengdar Tsai
Tsai Lab
Corresponding Author
J. Keith Joung
Massachusetts General Hospital and Harvard Medical School
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
CIRCLE-seq is a sensitive method for selective sequencing of Cas9-cleaved genomic DNA
molecules. Briefly, genomic DNA is circularized using a restriction-enzyme independent approach.
Successfully circularized molecules are enzymatically selected for by exonuclease treatment and
then treated with Cas9-gRNA complex. Only Cas9-linearized molecules can be subsequently
ligated to adapters for high-throughput sequencing.
Keywords
CIRCLE-seq
J.K.J. is a consultant for Horizon Discovery. J.K.J. has financial interests in Beacon Genomics,
Editas Medicine, Poseida Therapeutics, and Transposagen Biopharmaceuticals. J.K.J.’s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies. S.Q.T., M.J.A., and J.K.J. are scientific cofounders of Beacon Genomics.
This is a list of supplementary files associated with this preprint. Click to download.
- supplement0.pdf
Supplementary document 1: CIRCLE-seq protocol CIRCLE-seq protocol
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Posted 08 Nov, 2017
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Biotechnology
Associated Publications
CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
by Shengdar Q Tsai, Nhu T Nguyen, Jose Malagon-Lopez, +3
Nature Methods (02 May, 2017)
Method Article
Shengdar Tsai
Tsai Lab
Corresponding Author
J. Keith Joung
Massachusetts General Hospital and Harvard Medical School
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 08 Nov, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biotechnology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
CIRCLE-seq is a sensitive method for selective sequencing of Cas9-cleaved genomic DNA
molecules. Briefly, genomic DNA is circularized using a restriction-enzyme independent approach.
Successfully circularized molecules are enzymatically selected for by exonuclease treatment and
then treated with Cas9-gRNA complex. Only Cas9-linearized molecules can be subsequently
ligated to adapters for high-throughput sequencing.
Loading...
Associated Publications
CIRCLE-seq: a highly sensitive in vitro screen for genome-wide CRISPR–Cas9 nuclease off-targets
by Shengdar Q Tsai, Nhu T Nguyen, Jose Malagon-Lopez, +3
Nature Methods (02 May, 2017)