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Shengdar Tsai, J. Keith Joung, Nhu Nguyen, Jose Malagon-Lopez, Ved Topkar, Martin Aryee
J. Keith Joung
Massachusetts General Hospital and Harvard Medical School
CIRCLE-seq is a sensitive method for selective sequencing of Cas9-cleaved genomic DNA
molecules. Briefly, genomic DNA is circularized using a restriction-enzyme independent approach.
Successfully circularized molecules are enzymatically selected for by exonuclease treatment and
then treated with Cas9-gRNA complex. Only Cas9-linearized molecules can be subsequently
ligated to adapters for high-throughput sequencing.
J.K.J. is a consultant for Horizon Discovery. J.K.J. has financial interests in Beacon Genomics,
Editas Medicine, Poseida Therapeutics, and Transposagen Biopharmaceuticals. J.K.J.’s interests were reviewed and are managed by Massachusetts General Hospital and Partners HealthCare in accordance with their conflict of interest policies. S.Q.T., M.J.A., and J.K.J. are scientific cofounders of Beacon Genomics.
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