Hematopoietic stem/progenitor cell conversion from human pluripotent stem cells

doi:10.1038/protex.2017.045

This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.

Method Article

Rio Sugimura, George Daley, Scott Snapper, Alan Engelman, Gordon Keller, Andrea Ditadi, Ted N. Zhu, Patricia Sousa, Irene Wong, R. Grant Rowe, Mohan Malleshaiah, Erik Serrao, Jeremy A. Goettel, Yi-Fen Lu, Edroaldo Lummertz da Rocha, Clara Soria-Valles, Areum Han, Deepak Kumar Jha, Sergei Doulatov

Rio Sugimura

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

George Daley

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

Scott Snapper

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Alan Engelman

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Gordon Keller

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Andrea Ditadi

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Ted N. Zhu

Program in Computer Science, Harvard University, Cambridge, MA

Patricia Sousa

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Irene Wong

R. Grant Rowe

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Mohan Malleshaiah

Department of Systems Biology, Harvard Medical School, Boston, MA

Erik Serrao

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Jeremy A. Goettel

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Yi-Fen Lu

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Edroaldo Lummertz da Rocha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Clara Soria-Valles

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Areum Han

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Deepak Kumar Jha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Sergei Doulatov

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

DOI:

10.1038/protex.2017.045

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

This protocol is for hematopoietic stem/progenitor cell conversion from human pluripotent stem cells.

Keywords

human pluripotent stem cell

No competing financial interests declared.

This is a list of supplementary files associated with this preprint. Click to download.

supplement0.docx
Full Protocol Exchange protocol Hematopoietic stem/progenitor cell conversion from human pluripotent stem cells

Version History

CURRENT STATUS: Posted

Version 1

Posted 19 May, 2017

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Associated Publications

Haematopoietic stem and progenitor cells from human pluripotent stem cells
by Ryohichi Sugimura, Deepak Kumar Jha, Areum Han, +13
Nature (19 May, 2017)

Method Article

Rio Sugimura, George Daley, Scott Snapper, Alan Engelman, Gordon Keller, Andrea Ditadi, Ted N. Zhu, Patricia Sousa, Irene Wong, R. Grant Rowe, Mohan Malleshaiah, Erik Serrao, Jeremy A. Goettel, Yi-Fen Lu, Edroaldo Lummertz da Rocha, Clara Soria-Valles, Areum Han, Deepak Kumar Jha, Sergei Doulatov

Rio Sugimura

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

George Daley

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

Scott Snapper

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Alan Engelman

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Gordon Keller

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Andrea Ditadi

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Ted N. Zhu

Program in Computer Science, Harvard University, Cambridge, MA

Patricia Sousa

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Irene Wong

R. Grant Rowe

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Mohan Malleshaiah

Department of Systems Biology, Harvard Medical School, Boston, MA

Erik Serrao

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Jeremy A. Goettel

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Yi-Fen Lu

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Edroaldo Lummertz da Rocha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Clara Soria-Valles

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Areum Han

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Deepak Kumar Jha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Sergei Doulatov

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Version History

CURRENT STATUS: Posted

Version 1

Posted 19 May, 2017

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Biological techniques

DOI:

10.1038/protex.2017.045

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

This protocol is for hematopoietic stem/progenitor cell conversion from human pluripotent stem cells.

Associated Publications

Haematopoietic stem and progenitor cells from human pluripotent stem cells
by Ryohichi Sugimura, Deepak Kumar Jha, Areum Han, +13
Nature (19 May, 2017)

Method Article

Rio Sugimura, George Daley, Scott Snapper, Alan Engelman, Gordon Keller, Andrea Ditadi, Ted N. Zhu, Patricia Sousa, Irene Wong, R. Grant Rowe, Mohan Malleshaiah, Erik Serrao, Jeremy A. Goettel, Yi-Fen Lu, Edroaldo Lummertz da Rocha, Clara Soria-Valles, Areum Han, Deepak Kumar Jha, Sergei Doulatov

Rio Sugimura

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

George Daley

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

Scott Snapper

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Alan Engelman

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Gordon Keller

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Andrea Ditadi

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada

Ted N. Zhu

Program in Computer Science, Harvard University, Cambridge, MA

Patricia Sousa

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Irene Wong

R. Grant Rowe

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Mohan Malleshaiah

Department of Systems Biology, Harvard Medical School, Boston, MA

Erik Serrao

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Jeremy A. Goettel

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Yi-Fen Lu

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Edroaldo Lummertz da Rocha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Clara Soria-Valles

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Areum Han

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Deepak Kumar Jha

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Sergei Doulatov

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

DOI:

10.1038/protex.2017.045

Download PDF

License:

This work is licensed under a CC BY 4.0 License. Read Full License

Abstract

Keywords

human pluripotent stem cell

Procedure

Competing Interests

Supplementary Files

Comments

Version History

CURRENT STATUS: Posted

Version 1

No community comments so far

Metrics

Comments: 0

HTML Views: ...

Subject Areas

Biological techniques

Associated Publications

Haematopoietic stem and progenitor cells from human pluripotent stem cells

by Ryohichi Sugimura, Deepak Kumar Jha, Areum Han, +13

Nature (19 May, 2017)

Method Article

Rio Sugimura, George Daley, Scott Snapper, Alan Engelman, Gordon Keller, Andrea Ditadi, Ted N. Zhu, Patricia Sousa, Irene Wong, R. Grant Rowe, Mohan Malleshaiah, Erik Serrao, Jeremy A. Goettel, Yi-Fen Lu, Edroaldo Lummertz da Rocha, Clara Soria-Valles, Areum Han, Deepak Kumar Jha, Sergei Doulatov

Rio Sugimura

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

George Daley

Stem Cell Transplantation Program, Division of Pediatric Hematology and Oncology, Dana-Farber Cancer Institute

Corresponding Author

Scott Snapper

Department of Pediatrics, Division of Gastroenterology, Hepatology and Nutrition, Boston Children’s Hospital, Boston, MA

Alan Engelman

Department of Cancer Immunology and Virology, Dana-Farber Cancer Institute and Department of Medicine, Harvard Medical School, Boston, MA, 02215, USA

Gordon Keller

McEwen Centre for Regenerative Medicine, University Health Network, Toronto, Ontario M5G 1L7, Canada