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Method Article
Sergiu P. Pașca
Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we show the generation and assembly from human pluripotent stem (hPS) cells of subdomain-specific forebrain spheroids: human cortical spheroids (hCS) and human subpallial spheroids (hSS). This protocol describes hPS cell maintenance, neural spheroid generation, patterning and assembly to obtain functionally-integrated human forebrain 3D cultures.
Keywords
spheroid, spheroids
No competing financial interests declared.
*Fikri Birey & Jimena Andersen contributed equally to this work.
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Full Protocol Exchange protocol Generation and Assembly of Forebrain Spheroids
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CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2017
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Subject Areas
Biological techniques
Associated Publications
Assembly of functionally integrated human forebrain spheroids
by Fikri Birey, Jimena Andersen, Christopher D. Makinson, +12
Nature (27 April, 2017)
Method Article
Sergiu P. Pașca
Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Here we show the generation and assembly from human pluripotent stem (hPS) cells of subdomain-specific forebrain spheroids: human cortical spheroids (hCS) and human subpallial spheroids (hSS). This protocol describes hPS cell maintenance, neural spheroid generation, patterning and assembly to obtain functionally-integrated human forebrain 3D cultures.
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Associated Publications
Assembly of functionally integrated human forebrain spheroids
by Fikri Birey, Jimena Andersen, Christopher D. Makinson, +12
Nature (27 April, 2017)