This is a protocol preprint. Preprints are preliminary reports that have not undergone peer review. They should not be considered conclusive, used to inform clinical practice, or referenced by the media as validated information.
Method Article
Generation and Assembly of Forebrain Spheroids
Sergiu P. Pașca
Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Corresponding Author
License:
This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License
Here we show the generation and assembly from human pluripotent stem (hPS) cells of subdomain-specific forebrain spheroids: human cortical spheroids (hCS) and human subpallial spheroids (hSS). This protocol describes hPS cell maintenance, neural spheroid generation, patterning and assembly to obtain functionally-integrated human forebrain 3D cultures.
Keywords
spheroid, spheroids
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- AssemblyForebrainSpheroidsProtocolExchang.docx
Full Protocol Exchange protocol Generation and Assembly of Forebrain Spheroids
Version History
CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2017
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Subject Areas
Biological techniques
Associated Publications
Assembly of functionally integrated human forebrain spheroids
by Fikri Birey, Jimena Andersen, Christopher D. Makinson, +12
Nature (27 April, 2017)
Method Article
Generation and Assembly of Forebrain Spheroids
Sergiu P. Pașca
Department of Psychiatry and Behavioral Sciences, Center for Sleep Sciences and Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 27 Apr, 2017
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License
Here we show the generation and assembly from human pluripotent stem (hPS) cells of subdomain-specific forebrain spheroids: human cortical spheroids (hCS) and human subpallial spheroids (hSS). This protocol describes hPS cell maintenance, neural spheroid generation, patterning and assembly to obtain functionally-integrated human forebrain 3D cultures.
Associated Publications
Assembly of functionally integrated human forebrain spheroids
by Fikri Birey, Jimena Andersen, Christopher D. Makinson, +12
Nature (27 April, 2017)