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Method Article
Barcoding lentiviral Cre vectors for use in experiments involving downstream Tuba-seq analysis.
Zoe Rogers
Winslow Lab
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol outlines the procedure to massively barcode Lentiviral Cre vectors. This procedure was developed for use of the Tuba-seq pipeline to calculate tumor sizes in lentivirally inducible mouse models of cancer. The protocol accompanies Rogers et al, Nature Methods, published online May 22, 2017 (nmeth.4297).
Keywords
Tuba-seq, barcoding, lentivirus, Cre
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This is a preprint. It has not completed peer review.
Method Article
Barcoding lentiviral Cre vectors for use in experiments involving downstream Tuba-seq analysis.
Zoe Rogers
Winslow Lab
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 25 May, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY 4.0 License. Read Full License
This protocol outlines the procedure to massively barcode Lentiviral Cre vectors. This procedure was developed for use of the Tuba-seq pipeline to calculate tumor sizes in lentivirally inducible mouse models of cancer. The protocol accompanies Rogers et al, Nature Methods, published online May 22, 2017 (nmeth.4297).