This is a protocol preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Dan Dominissini
Dominissini Lab (Sheba, Tel-Aviv)
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
Keywords
2-O-methylation, RNA, Nm, epitranscriptome, modifications, 2prime-O-methylation
Figure 1
Figure 2
Figure 3
Figure 4
None
Loading...
Version History
CURRENT STATUS: Posted
Version 1
Posted 15 May, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Associated Publications
Method Article
Dan Dominissini
Dominissini Lab (Sheba, Tel-Aviv)
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 15 May, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
Figure 1
Figure 2
Figure 3
Figure 4
Loading...