Nm-seq
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
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Posted 15 May, 2017
Nm-seq
Posted 15 May, 2017
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
Figure 1
Figure 2
Figure 3
Figure 4
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