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Method Article
Nm-seq
Dan Dominissini
Dominissini Lab (Sheba, Tel-Aviv)
Corresponding Author
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The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
Keywords
2-O-methylation, RNA, Nm, epitranscriptome, modifications, 2prime-O-methylation
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This is a preprint. It has not completed peer review.
Method Article
Nm-seq
Dan Dominissini
Dominissini Lab (Sheba, Tel-Aviv)
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 15 May, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The ribose of RNA nucleotides can be 2'-O-methylated (Nm). Despite advances in high-throughput detection, its inert chemical nature still limits sensitivity and precludes mapping in messenger RNA (mRNA). We leveraged the differential reactivity of 2'-O-methylated and 2'-hydroxylated nucleosides to periodate oxidation to develop Nm-seq, a sensitive method for transcriptome-wide mapping of Nm with base precision. This protocol accompanies Dai et al, Nature Methods, published online May 15, 2017 (10.1038/nmeth.4294).
Figure 1
Figure 2
Figure 3
Figure 4