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Method Article
Daniel Bello-Gil
Group of Innate Immunity and Pathology of the Critical Patient, IDIBELL
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
Keywords
printed glycan array, glycochips, anti-carbohydrate antibodies
Authors declare no conflicting financial interests
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Associated Publications
Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?
by Nicolai Bovin, Polina Obukhova, Nadezhda Shilova, +6
Printed glycan array: antibodies as probed in undiluted serum and effects of dilution
by Nadezhda Shilova, Maxim Navakouski, Nailya Khasbiullina, +2
Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies
by Tatiana Pochechueva, Francis Jacob, Darlene R. Goldstein, +7
Method Article
Daniel Bello-Gil
Group of Innate Immunity and Pathology of the Critical Patient, IDIBELL
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
Loading...
Associated Publications
Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?
by Nicolai Bovin, Polina Obukhova, Nadezhda Shilova, +6
Printed glycan array: antibodies as probed in undiluted serum and effects of dilution
by Nadezhda Shilova, Maxim Navakouski, Nailya Khasbiullina, +2
Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies
by Tatiana Pochechueva, Francis Jacob, Darlene R. Goldstein, +7