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Method Article
Daniel Bello-Gil
Group of Innate Immunity and Pathology of the Critical Patient, IDIBELL
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
Keywords
printed glycan array, glycochips, anti-carbohydrate antibodies
Authors declare no conflicting financial interests
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Posted 23 Jun, 2017
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Associated Publications
Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?
by Nicolai Bovin, Polina Obukhova, Nadezhda Shilova, +6
Printed glycan array: antibodies as probed in undiluted serum and effects of dilution
by Nadezhda Shilova, Maxim Navakouski, Nailya Khasbiullina, +2
Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies
by Tatiana Pochechueva, Francis Jacob, Darlene R. Goldstein, +7
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Daniel Bello-Gil
Group of Innate Immunity and Pathology of the Critical Patient, IDIBELL
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
Authors declare no conflicting financial interests
Loading...
Associated Publications
Repertoire of human natural anti-glycan immunoglobulins. Do we have auto-antibodies?
by Nicolai Bovin, Polina Obukhova, Nadezhda Shilova, +6
Printed glycan array: antibodies as probed in undiluted serum and effects of dilution
by Nadezhda Shilova, Maxim Navakouski, Nailya Khasbiullina, +2
Comparison of printed glycan array, suspension array and ELISA in the detection of human anti-glycan antibodies
by Tatiana Pochechueva, Francis Jacob, Darlene R. Goldstein, +7