Determination of antibodies binding specificities by printed glycan array technology
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
Posted 23 Jun, 2017
Determination of antibodies binding specificities by printed glycan array technology
Posted 23 Jun, 2017
The present protocol is aimed to determine the natural anti-carbohydrate antibody repertoire (binding specificities) of alphaGal-KO mice. Glycochips (microchips printed with 577 different carbohydrate structures) were printed by Semiotik LLC (Moscow, Russia) using a collection of different amine-functionalized glycans and bacterial polysaccharides covalently coupled to N-hydroxysuccinimide-derivatized glass slides (slide H, Schott-Nexterion, Mainz, Germany). The glycan library included blood group antigens and some of the most frequently occurring terminal oligosaccharides, as well as core motifs of mammalian N- and O-linked glycoproteins and glycolipids, tumor-associated carbohydrate antigens, and polysaccharides from pathogenic bacteria with fully characterized structures (Zelinsky Institute of Organic chemistry, Moscow, Russia).
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