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Method Article
Generation of pure GABAergic neurons by transcription factor programming
Marius Wernig
Marius Wernig Lab, Stanford University
Corresponding Author
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Utilizing lineage-determining transcription factors, we previously developed a single-step method to generate glutamatergic neurons from human pluripotent stem cells. Now we show that transient expression of the transcription factors Ascl1 and Dlx2 induces exclusively GABAergic neurons from human pluripotent stem cells with a high degree of synaptic maturation. These AD-induced neuronal cells represent largely non-overlapping populations of GABAergic neurons expressing various subtype-specific markers and display a high degree of synaptic maturity after five weeks when cultured with glia. This protocol accompanies Yang et al, Nature Methods, published online May 15, 2017 (doi: 10.1038/nmeth.4291).
Keywords
human pluripotent stem cells, direct reprogramming, GABAergic neurons
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This is a preprint. It has not completed peer review.
Method Article
Generation of pure GABAergic neurons by transcription factor programming
Marius Wernig
Marius Wernig Lab, Stanford University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 15 May, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Utilizing lineage-determining transcription factors, we previously developed a single-step method to generate glutamatergic neurons from human pluripotent stem cells. Now we show that transient expression of the transcription factors Ascl1 and Dlx2 induces exclusively GABAergic neurons from human pluripotent stem cells with a high degree of synaptic maturation. These AD-induced neuronal cells represent largely non-overlapping populations of GABAergic neurons expressing various subtype-specific markers and display a high degree of synaptic maturity after five weeks when cultured with glia. This protocol accompanies Yang et al, Nature Methods, published online May 15, 2017 (doi: 10.1038/nmeth.4291).
Figure 1
Figure 2