Generation of pure GABAergic neurons by transcription factor programming
Utilizing lineage-determining transcription factors, we previously developed a single-step method to generate glutamatergic neurons from human pluripotent stem cells. Now we show that transient expression of the transcription factors Ascl1 and Dlx2 induces exclusively GABAergic neurons from human pluripotent stem cells with a high degree of synaptic maturation. These AD-induced neuronal cells represent largely non-overlapping populations of GABAergic neurons expressing various subtype-specific markers and display a high degree of synaptic maturity after five weeks when cultured with glia. This protocol accompanies Yang et al, Nature Methods, published online May 15, 2017 (doi: 10.1038/nmeth.4291).
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Posted 15 May, 2017
Generation of pure GABAergic neurons by transcription factor programming
Posted 15 May, 2017
Utilizing lineage-determining transcription factors, we previously developed a single-step method to generate glutamatergic neurons from human pluripotent stem cells. Now we show that transient expression of the transcription factors Ascl1 and Dlx2 induces exclusively GABAergic neurons from human pluripotent stem cells with a high degree of synaptic maturation. These AD-induced neuronal cells represent largely non-overlapping populations of GABAergic neurons expressing various subtype-specific markers and display a high degree of synaptic maturity after five weeks when cultured with glia. This protocol accompanies Yang et al, Nature Methods, published online May 15, 2017 (doi: 10.1038/nmeth.4291).
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