Preparation of peptidoglycan-depleted soluble microbial extract

doi:10.1038/nprot.2009.162

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Method Article

Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, Sung-Hee Kim, Jae-Hong Lim, Min-Sung Kim, Byung-Ha Oh, Jaesang Kim, Won-Jae Lee

Eun-Mi Ha

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Kyung-Ah Lee

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

You Yeong Seo

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Sung-Hee Kim

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Jae-Hong Lim

Beamline division, Pohang Accelerator Laboratory, Pohang, Kyungbuk, 790-784, South Korea.

Min-Sung Kim

Department of Biophysics & Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Byung-Ha Oh

Center for Biomolecular Recognition and Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, South Korea.

Jaesang Kim

Department of Life Science, Ewha Womans University, Seoul, South Korea

Won-Jae Lee

Division of Life and Pharmaceutical Science, Department of Life Science, Department of Bioinspired Science, and National Creative Research Initiative Center for Symbiosystem, Ewha Womans University, Seoul 120-750, South Korea.

DOI:

10.1038/nprot.2009.162

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Associated Publications

Coordination of multiple dual oxidase–regulatory pathways in responses to commensal and infectious microbes in drosophila gut
by Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, +4
Nature Immunology (11 June, 2009)

Method Article

Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, Sung-Hee Kim, Jae-Hong Lim, Min-Sung Kim, Byung-Ha Oh, Jaesang Kim, Won-Jae Lee

Eun-Mi Ha

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Kyung-Ah Lee

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

You Yeong Seo

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Sung-Hee Kim

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Jae-Hong Lim

Beamline division, Pohang Accelerator Laboratory, Pohang, Kyungbuk, 790-784, South Korea.

Min-Sung Kim

Department of Biophysics & Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Byung-Ha Oh

Center for Biomolecular Recognition and Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, South Korea.

Jaesang Kim

Department of Life Science, Ewha Womans University, Seoul, South Korea

Won-Jae Lee

Division of Life and Pharmaceutical Science, Department of Life Science, Department of Bioinspired Science, and National Creative Research Initiative Center for Symbiosystem, Ewha Womans University, Seoul 120-750, South Korea.

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CURRENT STATUS: Posted

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Subject Areas

Biochemistry

Computational biology and bioinformatics

Biological techniques

DOI:

10.1038/nprot.2009.162

Download PDF

Figure 1

Figure 2

Associated Publications

Coordination of multiple dual oxidase–regulatory pathways in responses to commensal and infectious microbes in drosophila gut
by Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, +4
Nature Immunology (11 June, 2009)

Method Article

Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, Sung-Hee Kim, Jae-Hong Lim, Min-Sung Kim, Byung-Ha Oh, Jaesang Kim, Won-Jae Lee

Eun-Mi Ha

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Kyung-Ah Lee

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

You Yeong Seo

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Sung-Hee Kim

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Jae-Hong Lim

Beamline division, Pohang Accelerator Laboratory, Pohang, Kyungbuk, 790-784, South Korea.

Min-Sung Kim

Department of Biophysics & Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Byung-Ha Oh

Center for Biomolecular Recognition and Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, South Korea.

Jaesang Kim

Department of Life Science, Ewha Womans University, Seoul, South Korea

Won-Jae Lee

Division of Life and Pharmaceutical Science, Department of Life Science, Department of Bioinspired Science, and National Creative Research Initiative Center for Symbiosystem, Ewha Womans University, Seoul 120-750, South Korea.

DOI:

10.1038/nprot.2009.162

Download PDF

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Introduction

Reagents

Equipment

Procedure

Timing

Critical Steps

Anticipated Results

References

Acknowledgements

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CURRENT STATUS: Posted

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Subject Areas

Biochemistry

Computational biology and bioinformatics

Biological techniques

Associated Publications

Coordination of multiple dual oxidase–regulatory pathways in responses to commensal and infectious microbes in drosophila gut

by Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, +4

Nature Immunology (11 June, 2009)

Method Article

Eun-Mi Ha, Kyung-Ah Lee, You Yeong Seo, Sung-Hee Kim, Jae-Hong Lim, Min-Sung Kim, Byung-Ha Oh, Jaesang Kim, Won-Jae Lee

Eun-Mi Ha

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Kyung-Ah Lee

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

You Yeong Seo

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Sung-Hee Kim

Ewha Womans University and National Creative Research Center for Symbiosystem, Seoul, South Korea

Jae-Hong Lim

Beamline division, Pohang Accelerator Laboratory, Pohang, Kyungbuk, 790-784, South Korea.

Min-Sung Kim

Department of Biophysics & Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205.

Byung-Ha Oh

Center for Biomolecular Recognition and Division of Molecular and Life Science, Pohang University of Science and Technology, Pohang, Kyungbuk, 790-784, South Korea.

Jaesang Kim

Department of Life Science, Ewha Womans University, Seoul, South Korea

Won-Jae Lee

Division of Life and Pharmaceutical Science, Department of Life Science, Department of Bioinspired Science, and National Creative Research Initiative Center for Symbiosystem, Ewha Womans University, Seoul 120-750, South Korea.

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