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Method Article
J Christopher Love
Koch Institute, Broad Institute, Ragon Institute
Corresponding Author
Alex Shalek
MIT, Broad Institute, Ragon Institute
Corresponding Author
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well extensively and use it to profile thousands of primary human macrophages exposed to tuberculosis.
Keywords
high-throughput, single-cell RNA-Seq, microwells
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The authors declare competing financial interests: details are available in the online version of the paper.
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Associated Publications
Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
by Todd M Gierahn, Marc H Wadsworth, Travis K Hughes, +6
Nature Methods (07 February, 2017)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
J Christopher Love
Koch Institute, Broad Institute, Ragon Institute
Corresponding Author
Alex Shalek
MIT, Broad Institute, Ragon Institute
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 14 Mar, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Single-cell RNA-seq can precisely resolve cellular states, but applying this method to low-input samples is challenging. Here, we present Seq-Well, a portable, low-cost platform for massively parallel single-cell RNA-seq. Barcoded mRNA capture beads and single cells are sealed in an array of subnanoliter wells using a semipermeable membrane, enabling efficient cell lysis and transcript capture. We characterize Seq-Well extensively and use it to profile thousands of primary human macrophages exposed to tuberculosis.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
Figure 6
Figure 7
Figure 8
Figure 9
Figure 10
Figure 11
Figure 12
Figure 13
Figure 14
Figure 15
Figure 16
Figure 17
Figure 18
Figure 19
Figure 20
Figure 21
Figure 22
Figure 23
Figure 24
Figure 25
Figure 26
Figure 27
Figure 28
Figure 29
Figure 30
The authors declare competing financial interests: details are available in the online version of the paper.
Loading...
Associated Publications
Seq-Well: portable, low-cost RNA sequencing of single cells at high throughput
by Todd M Gierahn, Marc H Wadsworth, Travis K Hughes, +6
Nature Methods (07 February, 2017)