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Method Article
Combinatorial CRISPR-Cas9 Knockout Screen
Dongxin Zhao
Mali Lab
Corresponding Author
John Paul Shen
Ideker Lab
Corresponding Author
Roman Sasik
UCSD Center for Computational Biology & Bioinformatics
Corresponding Author
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This work is licensed under a CC BY 4.0 License. Read Full License
Simultaneous mutation of two genes can produce a phenotype that is unexpected in light of each mutation’s individual effect. This phenomenon, known as genetic interaction, identifies an underlying functional relationship between the genes, such as contributions to the same protein complex or pathway. Mapping these functional relationships has broad applicability for advancing our fundamental understanding of biological systems and also has direct implications for cancer therapeutic development. Here we describe a method using the CRISPR-Cas9 system to knockout pairs of genes, enabling high-throughput, systematic mapping of these genetic interaction networks in human cells. This protocol accompanies Shen et al (Nature Methods, publishing online 20 March, 2017; 10.038/nmeth.4225); it was added to the manuscript after formal peer review, as an aid to users.
Keywords
CRISPR, Genetic Interaction, Synthetic Lethal, Cancer
Figure 1
Yves Matthess
commented on 31 March, 2020
Dear Dr. Mali, did you integrate Cas9 into AAVS1? Did you test Cas9 activity? THX.
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This is a preprint. It has not completed peer review.
Method Article
Combinatorial CRISPR-Cas9 Knockout Screen
Dongxin Zhao
Mali Lab
Corresponding Author
John Paul Shen
Ideker Lab
Corresponding Author
Roman Sasik
UCSD Center for Computational Biology & Bioinformatics
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 20 Mar, 2017
Community comments: 1
Metrics
Comments: 1
HTML Views: ...
License:
This work is licensed under a CC BY 4.0 License. Read Full License
Simultaneous mutation of two genes can produce a phenotype that is unexpected in light of each mutation’s individual effect. This phenomenon, known as genetic interaction, identifies an underlying functional relationship between the genes, such as contributions to the same protein complex or pathway. Mapping these functional relationships has broad applicability for advancing our fundamental understanding of biological systems and also has direct implications for cancer therapeutic development. Here we describe a method using the CRISPR-Cas9 system to knockout pairs of genes, enabling high-throughput, systematic mapping of these genetic interaction networks in human cells. This protocol accompanies Shen et al (Nature Methods, publishing online 20 March, 2017; 10.038/nmeth.4225); it was added to the manuscript after formal peer review, as an aid to users.
Figure 1
Yves Matthess
commented on 31 March, 2020
Dear Dr. Mali, did you integrate Cas9 into AAVS1? Did you test Cas9 activity? THX.