Using MSFragger for ultrafast database searching
Database search has long been the preferred method for peptide identification in shotgun proteomics. While a wide range of database search tools exist and excel for conventional workflows, they are too slow for open database searching. To make open searching practical for routine analysis, we developed a novel database search tool called MSFragger that is over 100 times faster than most existing tools.
This protocol demonstrates how to perform database searching using MSFragger. Up-to-date downloads of MSFragger and associated documentation can be found at "nesvilab.org/software.html":http://nesvilab.org/software.html.
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This is a list of supplementary files associated with this preprint. Click to download.
MSFraggerParamGuide Guide to Search Parameters
Posted 10 Apr, 2017
Using MSFragger for ultrafast database searching
Posted 10 Apr, 2017
Database search has long been the preferred method for peptide identification in shotgun proteomics. While a wide range of database search tools exist and excel for conventional workflows, they are too slow for open database searching. To make open searching practical for routine analysis, we developed a novel database search tool called MSFragger that is over 100 times faster than most existing tools.
This protocol demonstrates how to perform database searching using MSFragger. Up-to-date downloads of MSFragger and associated documentation can be found at "nesvilab.org/software.html":http://nesvilab.org/software.html.
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