Optimized transfection protocol for efficient in vitro non-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19)

doi:10.1038/nprot.2009.78

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Method Article

Optimized transfection protocol for efficient in vitro non-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19)

Astrid Subrizi, Marjo Yliperttula, Lorenzo Tibaldi, Etienne Schacht, Peter Dubruel, Alain Joliot, Arto Urtti

Astrid Subrizi

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland; Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Marjo Yliperttula

Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Lorenzo Tibaldi

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Etienne Schacht

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Peter Dubruel

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Alain Joliot

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Arto Urtti

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

DOI:

10.1038/nprot.2009.78

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This is a preprint. It has not completed peer review.

Method Article

Optimized transfection protocol for efficient in vitro non-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19)

Astrid Subrizi, Marjo Yliperttula, Lorenzo Tibaldi, Etienne Schacht, Peter Dubruel, Alain Joliot, Arto Urtti

Astrid Subrizi

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland; Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Marjo Yliperttula

Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Lorenzo Tibaldi

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Etienne Schacht

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Peter Dubruel

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Alain Joliot

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Arto Urtti

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

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CURRENT STATUS: Posted

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Subject Areas

Biological techniques

Genetics

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Biochemistry

Nanoscience and technology

DOI:

10.1038/nprot.2009.78

Download PDF

Figure 1

Figure 2

Figure 3

Method Article

Optimized transfection protocol for efficient in vitro non-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19)

Astrid Subrizi, Marjo Yliperttula, Lorenzo Tibaldi, Etienne Schacht, Peter Dubruel, Alain Joliot, Arto Urtti

Astrid Subrizi

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland; Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Marjo Yliperttula

Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Lorenzo Tibaldi

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Etienne Schacht

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Peter Dubruel

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Alain Joliot

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Arto Urtti

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

DOI:

10.1038/nprot.2009.78

Download PDF

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CURRENT STATUS: Posted

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Subject Areas

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Nanoscience and technology

Learn more about our company.

This is a preprint. It has not completed peer review.

Method Article

Optimized transfection protocol for efficient in vitro non-viral polymeric gene delivery to human retinal pigment epithelial cells (ARPE-19)

Astrid Subrizi, Marjo Yliperttula, Lorenzo Tibaldi, Etienne Schacht, Peter Dubruel, Alain Joliot, Arto Urtti

Astrid Subrizi

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland; Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Marjo Yliperttula

Division of Biopharmaceutics and Pharmacokinetics, Faculty of Pharmacy, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

Lorenzo Tibaldi

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Etienne Schacht

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Peter Dubruel

Department Organic Chemistry, Ghent University, Krijgslaan 281 S-4, 9000, Ghent, Belgium

Alain Joliot

Ecole Normale Superieure, Homeoprotein cell biology, CNRS UMR 8542, 46,rue d'Ulm, 75005 Paris, France

Arto Urtti

Centre for Drug Research, University of Helsinki, Viikinkaari 5 E, 00790 Helsinki, Finland

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