Isolation of exosomal RNA from cell culture supernatant using the System Biosciences Exoquick Seramir tissue culture kit

doi:10.1038/protex.2017.085

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Method Article

Saumya Das, Louise C. Laurent, Tushar Patel, Kirsty Danielson, Bridget Simonson, Srimeenakshi Srinivasan, Irene K. Yan

Saumya Das

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Corresponding Author

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Tushar Patel

Mayo Clinic Florida, Jacksonville, FL, USA

Corresponding Author

Kirsty Danielson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Bridget Simonson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Irene K. Yan

Mayo Clinic Florida, Jacksonville, FL, USA

DOI:

10.1038/protex.2017.085

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License:

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This protocol describes how to isolate exosomal RNA from cell culture supernatant using the Exoquick Seramir tissue culture kit.

Keywords

exRNA, extracellular vesicles, exosomes, RNA isolation, Exoquick, SeraMir

The authors declare no competing financial interests.

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Cell biology

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Method Article

Saumya Das, Louise C. Laurent, Tushar Patel, Kirsty Danielson, Bridget Simonson, Srimeenakshi Srinivasan, Irene K. Yan

Saumya Das

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Corresponding Author

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Tushar Patel

Mayo Clinic Florida, Jacksonville, FL, USA

Corresponding Author

Kirsty Danielson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Bridget Simonson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Irene K. Yan

Mayo Clinic Florida, Jacksonville, FL, USA

Version History

CURRENT STATUS: Posted

Version 1

Posted 16 Jun, 2017

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Molecular Biology

DOI:

10.1038/protex.2017.085

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol describes how to isolate exosomal RNA from cell culture supernatant using the Exoquick Seramir tissue culture kit.

Method Article

Saumya Das, Louise C. Laurent, Tushar Patel, Kirsty Danielson, Bridget Simonson, Srimeenakshi Srinivasan, Irene K. Yan

Saumya Das

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Corresponding Author

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Tushar Patel

Mayo Clinic Florida, Jacksonville, FL, USA

Corresponding Author

Kirsty Danielson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Bridget Simonson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Irene K. Yan

Mayo Clinic Florida, Jacksonville, FL, USA

DOI:

10.1038/protex.2017.085

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

exRNA, extracellular vesicles, exosomes, RNA isolation, Exoquick, SeraMir

Introduction

Reagents

Equipment

Procedure

Troubleshooting

Acknowledgements

Competing Interests

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CURRENT STATUS: Posted

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Subject Areas

Cell biology

Biological techniques

Molecular Biology

Method Article

Saumya Das, Louise C. Laurent, Tushar Patel, Kirsty Danielson, Bridget Simonson, Srimeenakshi Srinivasan, Irene K. Yan

Saumya Das

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Corresponding Author

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Tushar Patel

Mayo Clinic Florida, Jacksonville, FL, USA

Corresponding Author

Kirsty Danielson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Bridget Simonson

Cardiovascular Research Center, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Irene K. Yan

Mayo Clinic Florida, Jacksonville, FL, USA

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Subject Areas

Cell biology

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Molecular Biology