Isolation of exosomal RNA from urine using ultracentrifugation and the Qiagen miRNeasy Micro kit

doi:10.1038/protex.2017.084

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Method Article

Louise C. Laurent, Srimeenakshi Srinivasan

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

DOI:

10.1038/protex.2017.084

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This protocol describes how to isolate exosomal RNA from urine by using ultracentrifugation for exosomal enrichment followed by the Qiagen miRNeasy Micro kit for RNA isolation.

Keywords

exRNA, extracellular vesicles, exosomes, urine, ultracentrifugation, RNA isolation, miRNeasy kit

The authors declare no competing financial interests.

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An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Srimeenakshi Srinivasan

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Version History

CURRENT STATUS: Posted

Version 1

Posted 16 Jun, 2017

No community comments so far

MetricsComments: 0HTML Views: ...

Subject Areas

Cell biology

Biological techniques

Molecular Biology

DOI:

10.1038/protex.2017.084

Download PDF

The full text of this article is available to read as a PDF.

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

This protocol describes how to isolate exosomal RNA from urine by using ultracentrifugation for exosomal enrichment followed by the Qiagen miRNeasy Micro kit for RNA isolation.

The authors declare no competing financial interests.

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Srimeenakshi Srinivasan

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

DOI:

10.1038/protex.2017.084

Download PDF

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Abstract

Keywords

exRNA, extracellular vesicles, exosomes, urine, ultracentrifugation, RNA isolation, miRNeasy kit

Introduction

Reagents

Equipment

Procedure

Acknowledgements

Competing Interests

Comments

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CURRENT STATUS: Posted

Version 1

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Subject Areas

Cell biology

Biological techniques

Molecular Biology

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Louise C. Laurent, Srimeenakshi Srinivasan

Louise C. Laurent

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

Corresponding Author

Srimeenakshi Srinivasan

Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA

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Subject Areas

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DOI:

10.1038/protex.2017.084

Download PDF

License

License:

This work is licensed under a CC BY-NC 3.0 License. Read Full License

Declarations

Abstract

Introduction

Reagents

Equipment

Procedure

Acknowledgements

Competing Interests

Comments

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