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Method Article
Isolation of exosomal RNA from urine using ultracentrifugation and the Qiagen miRNeasy Micro kit
Louise C. Laurent
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes how to isolate exosomal RNA from urine by using ultracentrifugation for exosomal enrichment followed by the Qiagen miRNeasy Micro kit for RNA isolation.
Keywords
exRNA, extracellular vesicles, exosomes, urine, ultracentrifugation, RNA isolation, miRNeasy kit
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This is a preprint. It has not completed peer review.
Method Article
Isolation of exosomal RNA from urine using ultracentrifugation and the Qiagen miRNeasy Micro kit
Louise C. Laurent
Department of Reproductive Medicine, University of California, San Diego, La Jolla, CA, USA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 16 Jun, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes how to isolate exosomal RNA from urine by using ultracentrifugation for exosomal enrichment followed by the Qiagen miRNeasy Micro kit for RNA isolation.