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Method Article
Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals
Tamir Gonen
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, USA
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
Keywords
Biochemistry, Biology, Chemical biology, CryoEM, Crystallography, Electron microscopy, MicroED, Proteins, Structural biology, XFEL
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Associated Publications
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
by M Jason de la Cruz, Johan Hattne, Dan Shi, +12
Nature Methods (14 February, 2017)
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This is a preprint. It has not completed peer review.
Method Article
Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals
Tamir Gonen
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, USA
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 14 Feb, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
Associated Publications
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
by M Jason de la Cruz, Johan Hattne, Dan Shi, +12
Nature Methods (14 February, 2017)