Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
Posted 14 Feb, 2017
Micro- and nanocrystal preparation for MicroED and XFEL serial crystallography by fragmentation of imperfect crystals
Posted 14 Feb, 2017
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
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