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Method Article
Tamir Gonen
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, USA
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
Keywords
Biochemistry, Biology, Chemical biology, CryoEM, Crystallography, Electron microscopy, MicroED, Proteins, Structural biology, XFEL
The authors declare no competing financial interests.
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Associated Publications
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
by M Jason de la Cruz, Johan Hattne, Dan Shi, +12
Nature Methods (14 February, 2017)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Tamir Gonen
Howard Hughes Medical Institute, Janelia Research Campus, Ashburn, Virginia 20147, USA
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 14 Feb, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes different techniques to fragment protein crystals for high-resolution structure determination by MicroED and serial crystallography at an X-ray free electron laser. These techniques involve agitation of the crystal of interest: 1) by vigorous pipetting, 2) by sonication, and 3) by vortexing. Aside from setup, the time needed to perform these steps ranges from seconds to minutes.
The authors declare no competing financial interests.
Loading...
Associated Publications
Atomic-resolution structures from fragmented protein crystals with the cryoEM method MicroED
by M Jason de la Cruz, Johan Hattne, Dan Shi, +12
Nature Methods (14 February, 2017)