Well-defined matrices for the expansion of intestinal stem cells and organoids
Stem-cell derived organoids approximate organ development, structure and function with unprecedented fidelity, opening new avenues in basic research, disease modeling and cell-based therapies. However, their potential is limited by the ill-defined matrices used for their culture. Here, we describe well-defined poly(ethylene glycol)-based matrices for the culture of intestinal stem cells and organoids. We provide a protocol for the synthesis and purification of hydrogel precursors, as well as instructions for hydrogel formation and mechanical characterization. Finally, guidelines for intestinal stem cell expansion and organoid formation within these matrices are provided.
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Posted 12 Jan, 2017
Well-defined matrices for the expansion of intestinal stem cells and organoids
Posted 12 Jan, 2017
Stem-cell derived organoids approximate organ development, structure and function with unprecedented fidelity, opening new avenues in basic research, disease modeling and cell-based therapies. However, their potential is limited by the ill-defined matrices used for their culture. Here, we describe well-defined poly(ethylene glycol)-based matrices for the culture of intestinal stem cells and organoids. We provide a protocol for the synthesis and purification of hydrogel precursors, as well as instructions for hydrogel formation and mechanical characterization. Finally, guidelines for intestinal stem cell expansion and organoid formation within these matrices are provided.
Figure 1
Figure 2
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