This is a protocol preprint. Preprints are preliminary reports that have not undergone peer review. They should not be considered conclusive, used to inform clinical practice, or referenced by the media as validated information.
Method Article
Well-defined matrices for the expansion of intestinal stem cells and organoids
Matthias P. Lutolf
LSCB, EPFL
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Declarations:
Stem-cell derived organoids approximate organ development, structure and function with unprecedented fidelity, opening new avenues in basic research, disease modeling and cell-based therapies. However, their potential is limited by the ill-defined matrices used for their culture. Here, we describe well-defined poly(ethylene glycol)-based matrices for the culture of intestinal stem cells and organoids. We provide a protocol for the synthesis and purification of hydrogel precursors, as well as instructions for hydrogel formation and mechanical characterization. Finally, guidelines for intestinal stem cell expansion and organoid formation within these matrices are provided.
Keywords
Stem cells, organoids, biomechanics, mechanobiology, extracellular matrix, matrix stiffness, synthetic matrices, three-dimensional culture
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Method Article
Well-defined matrices for the expansion of intestinal stem cells and organoids
Matthias P. Lutolf
LSCB, EPFL
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 12 Jan, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Declarations:
Stem-cell derived organoids approximate organ development, structure and function with unprecedented fidelity, opening new avenues in basic research, disease modeling and cell-based therapies. However, their potential is limited by the ill-defined matrices used for their culture. Here, we describe well-defined poly(ethylene glycol)-based matrices for the culture of intestinal stem cells and organoids. We provide a protocol for the synthesis and purification of hydrogel precursors, as well as instructions for hydrogel formation and mechanical characterization. Finally, guidelines for intestinal stem cell expansion and organoid formation within these matrices are provided.
Figure 1
Figure 2