A protocol for the differentiation of brown adipose progenitors derived from human induced pluripotent stem cells at a high efficiency with no gene transfer
Human induced pluripotent stem cells (hiPSC) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, BAPs derived from hiPSCs (hiPSC-BAPs) display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this features are unknown and hamper their use both in cell based therapy and basic research. We describe the steps in details to derive BAPs from hiPSCs and to differentiate hiPSC-BAPs at a high rate into mature brown adipocytes. hiPSC-BAPs can be subcultured to produce large amount of brown/brite adipocytes and cryopreserved. This model offers a platform for the identification of pathways governing the earliest steps of human adipocyte development as well as an abundant source of cells for cell therapy approaches.
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Posted 22 Sep, 2016
A protocol for the differentiation of brown adipose progenitors derived from human induced pluripotent stem cells at a high efficiency with no gene transfer
Posted 22 Sep, 2016
Human induced pluripotent stem cells (hiPSC) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, BAPs derived from hiPSCs (hiPSC-BAPs) display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this features are unknown and hamper their use both in cell based therapy and basic research. We describe the steps in details to derive BAPs from hiPSCs and to differentiate hiPSC-BAPs at a high rate into mature brown adipocytes. hiPSC-BAPs can be subcultured to produce large amount of brown/brite adipocytes and cryopreserved. This model offers a platform for the identification of pathways governing the earliest steps of human adipocyte development as well as an abundant source of cells for cell therapy approaches.
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