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Method Article
Christian Dani
Dani's Lab, University of Côte d'Azur Nice
Corresponding Author
License:
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Human induced pluripotent stem cells (hiPSC) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, BAPs derived from hiPSCs (hiPSC-BAPs) display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this features are unknown and hamper their use both in cell based therapy and basic research. We describe the steps in details to derive BAPs from hiPSCs and to differentiate hiPSC-BAPs at a high rate into mature brown adipocytes. hiPSC-BAPs can be subcultured to produce large amount of brown/brite adipocytes and cryopreserved. This model offers a platform for the identification of pathways governing the earliest steps of human adipocyte development as well as an abundant source of cells for cell therapy approaches.
Keywords
human induced pluripotent stem cells, brown adipocytes, adipocytes, differentiation, adipose progenitors
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The authors declare no copeting financial interests
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Associated Publications
Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity
by Anne-Laure Hafner, Julian Contet, Christophe Ravaud, +9
Scientific Reports (13 September, 2016)
An open repository of community-contributed protocols sponsored by Nature Research.
Learn more about Protocol Exchange
Method Article
Christian Dani
Dani's Lab, University of Côte d'Azur Nice
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 22 Sep, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Human induced pluripotent stem cells (hiPSC) show great promise for obesity treatment as they represent an unlimited source of brown/brite adipose progenitors (BAPs). However, BAPs derived from hiPSCs (hiPSC-BAPs) display a low adipogenic capacity compared to adult-BAPs when maintained in a traditional adipogenic cocktail. The reasons of this features are unknown and hamper their use both in cell based therapy and basic research. We describe the steps in details to derive BAPs from hiPSCs and to differentiate hiPSC-BAPs at a high rate into mature brown adipocytes. hiPSC-BAPs can be subcultured to produce large amount of brown/brite adipocytes and cryopreserved. This model offers a platform for the identification of pathways governing the earliest steps of human adipocyte development as well as an abundant source of cells for cell therapy approaches.
Figure 1
Figure 2
The authors declare no copeting financial interests
Loading...
Associated Publications
Brown-like adipose progenitors derived from human induced pluripotent stem cells: Identification of critical pathways governing their adipogenic capacity
by Anne-Laure Hafner, Julian Contet, Christophe Ravaud, +9
Scientific Reports (13 September, 2016)