Direct reprogramming of mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs)
Here, we describe how to reprogram mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs) by lentiviral transduction of four transcription factors. First, MEFs are isolated from limbs of E12.5 reporter mice. Second, lentivirus is generated to overexpress transcription factors. Finally, reprogrammed iRECs are selected by flow cytometry.
Posted 11 Jan, 2017
Direct reprogramming of mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs)
Posted 11 Jan, 2017
Here, we describe how to reprogram mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs) by lentiviral transduction of four transcription factors. First, MEFs are isolated from limbs of E12.5 reporter mice. Second, lentivirus is generated to overexpress transcription factors. Finally, reprogrammed iRECs are selected by flow cytometry.
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