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Method Article
Sebastian Arnold
Sebastian Arnold Lab (Freiburg)
Corresponding Author
Soeren Lienkamp
Soeren Lienkamp Lab (Freiburg)
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Here, we describe how to reprogram mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs) by lentiviral transduction of four transcription factors. First, MEFs are isolated from limbs of E12.5 reporter mice. Second, lentivirus is generated to overexpress transcription factors. Finally, reprogrammed iRECs are selected by flow cytometry.
Keywords
iREC, direct reprogramming, renal tubule cells, fibroblasts
The authors declare that the following financial interest exists: patent application is pending (EPA 16152655.3).
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Associated Publications
Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors
by Michael M. Kaminski, Jelena Tosic, Catena Kresbach, +10
Nature Cell Biology (11 January, 2017)
Method Article
Sebastian Arnold
Sebastian Arnold Lab (Freiburg)
Corresponding Author
Soeren Lienkamp
Soeren Lienkamp Lab (Freiburg)
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 11 Jan, 2017
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
Here, we describe how to reprogram mouse embryonic fibroblasts into renal tubular epithelial cells (iRECs) by lentiviral transduction of four transcription factors. First, MEFs are isolated from limbs of E12.5 reporter mice. Second, lentivirus is generated to overexpress transcription factors. Finally, reprogrammed iRECs are selected by flow cytometry.
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Associated Publications
Direct reprogramming of fibroblasts into renal tubular epithelial cells by defined transcription factors
by Michael M. Kaminski, Jelena Tosic, Catena Kresbach, +10
Nature Cell Biology (11 January, 2017)