This is a preprint, a preliminary version of a manuscript that has not completed peer review at a journal. Research Square does not conduct peer review prior to posting preprints. The posting of a preprint on this server should not be interpreted as an endorsement of its validity or suitability for dissemination as established information or for guiding clinical practice.
Method Article
Stripping protocol for Affymetrix tiling gene chips
Kate McInnerney
commented on 06 April, 2009
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
commented on 30 June, 2011
good
Kate McInnerney
commented on 06 April, 2009
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
commented on 30 June, 2011
good
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Dec, 2008
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
Learn more about our company.
This is a preprint. It has not completed peer review.
Method Article
Stripping protocol for Affymetrix tiling gene chips
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 10 Dec, 2008
Community comments: 4
Metrics
Comments: 4
HTML Views: ...
Kate McInnerney
commented on 06 April, 2009
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
commented on 30 June, 2011
good
Kate McInnerney
commented on 06 April, 2009
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
commented on 30 June, 2011
good