Stripping protocol for Affymetrix tiling gene chips
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
good
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
good
Posted 10 Dec, 2008
Stripping protocol for Affymetrix tiling gene chips
Posted 10 Dec, 2008
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
good
In tests, this protocol also worked exceptionally well with Affy expression arrays, with little loss of signal intensity or gain of background. After three hybridizations (two strippings) the chip cassettes begin to fail, with sample leakage from the cassette face. Decreased stripping temps might be possible for cRNA hybs.
good
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