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Method Article
Dual Activation of CD95/CXCR4 in HEK293T cells by Abscisic Acid- and Gibberellin-inducible dCas9s (Transient Transfection)
Lei S Qi
Stanford University
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
The use of dCas9-based transcriptional activators and repressors facilitates manipulation of endogenous gene expression. However, precise temporal control of their activities and independent control of discrete gene targets in the same cell remain difficult. Here, we describe a method for utilizing orthogonal and small-molecule inducible dCas9-based transcriptional regulators for independently activating expression of CD95 and CXCR4 in HEK293T cells via transient transfection. This protocol can be easily adapted for different gene targets by using alternative sgRNAs, providing a customizable platform for flexible gene regulation.
Keywords
CRISPR, Cas9, Gene regulation, Synthetic biology, Inducible
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This is a preprint. It has not completed peer review.
Method Article
Dual Activation of CD95/CXCR4 in HEK293T cells by Abscisic Acid- and Gibberellin-inducible dCas9s (Transient Transfection)
Lei S Qi
Stanford University
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 25 Oct, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Molecular Biology
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
The use of dCas9-based transcriptional activators and repressors facilitates manipulation of endogenous gene expression. However, precise temporal control of their activities and independent control of discrete gene targets in the same cell remain difficult. Here, we describe a method for utilizing orthogonal and small-molecule inducible dCas9-based transcriptional regulators for independently activating expression of CD95 and CXCR4 in HEK293T cells via transient transfection. This protocol can be easily adapted for different gene targets by using alternative sgRNAs, providing a customizable platform for flexible gene regulation.
Figure 1
Figure 2