Improved exosome isolation by sucrose gradient fractionation of ultracentrifuged crude exosome pellets
This protocol describes a method for purifying exosomes using sucrose gradient centrifugation of crude exosome pellets prepared by differential ultracentrifugation. Exosomes isolated by differential ultracentrifugation alone contain both exosomes and aggregated protein and nucleic acid contaminants. Sucrose gradient centrifugation effectively separates exosomes and other extracellular vesicles from these contaminants.
Figure 1
Posted 23 Aug, 2016
Improved exosome isolation by sucrose gradient fractionation of ultracentrifuged crude exosome pellets
Posted 23 Aug, 2016
This protocol describes a method for purifying exosomes using sucrose gradient centrifugation of crude exosome pellets prepared by differential ultracentrifugation. Exosomes isolated by differential ultracentrifugation alone contain both exosomes and aggregated protein and nucleic acid contaminants. Sucrose gradient centrifugation effectively separates exosomes and other extracellular vesicles from these contaminants.
Figure 1
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