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Method Article
Ni-Ting Chiou
Department of Microbiology & Immunology, Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA 94143
Corresponding Author
K. Mark Ansel
Department of Microbiology & Immunology, Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA 94143
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method for purifying exosomes using sucrose gradient centrifugation of crude exosome pellets prepared by differential ultracentrifugation. Exosomes isolated by differential ultracentrifugation alone contain both exosomes and aggregated protein and nucleic acid contaminants. Sucrose gradient centrifugation effectively separates exosomes and other extracellular vesicles from these contaminants.
Keywords
exosome; extracellular vesicle; sucrose gradient; ultracentrifugation
Figure 1
The authors declare no competing financial interests.
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Posted 23 Aug, 2016
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Method Article
Ni-Ting Chiou
Department of Microbiology & Immunology, Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA 94143
Corresponding Author
K. Mark Ansel
Department of Microbiology & Immunology, Sandler Asthma Basic Research Center, University of California San Francisco, San Francisco, CA 94143
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Aug, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
Subject Areas
Biological techniques
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
This protocol describes a method for purifying exosomes using sucrose gradient centrifugation of crude exosome pellets prepared by differential ultracentrifugation. Exosomes isolated by differential ultracentrifugation alone contain both exosomes and aggregated protein and nucleic acid contaminants. Sucrose gradient centrifugation effectively separates exosomes and other extracellular vesicles from these contaminants.
Figure 1
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