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Method Article
DSBCapture: in situ single-nucleotide resolution DNA double-strand break mapping
Shankar Balasubramanian
Department of Chemistry, University of Cambridge
Corresponding Author
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This work is licensed under a CC BY-NC 3.0 License. Read Full License
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
- Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
Keywords
double-strand DNA break, double-strand DNA break mapping, next generation sequencing, molecular biology, DNA damage, G-quadruplex, Transcription
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Version 1 of Protocol Version 1 of Protocol Procedure steps 109 & 110 modified - Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
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Associated Publications
DSBCapture: in situ capture and sequencing of DNA breaks
by Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, +3
Nature Methods (15 August, 2016)
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This is a preprint. It has not completed peer review.
Method Article
DSBCapture: in situ single-nucleotide resolution DNA double-strand break mapping
Shankar Balasubramanian
Department of Chemistry, University of Cambridge
Corresponding Author
Badges
Prescreen
This manuscript passed our Prescreen assessment
Complete author information
Appropriate declaration statements
Potential risks to human health
Prescreen
History
CURRENT STATUS: Posted
Version 1
Posted 23 Aug, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
- Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
Figure 1
Figure 2
Figure 3
Associated Publications
DSBCapture: in situ capture and sequencing of DNA breaks
by Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, +3
Nature Methods (15 August, 2016)