DSBCapture: in situ single-nucleotide resolution DNA double-strand break mapping
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
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Version 1 of Protocol Version 1 of Protocol Procedure steps 109 & 110 modified - Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
Posted 23 Aug, 2016
DSBCapture: in situ single-nucleotide resolution DNA double-strand break mapping
Posted 23 Aug, 2016
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
Figure 1
Figure 2
Figure 3
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