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Method Article
Shankar Balasubramanian
Department of Chemistry, University of Cambridge
Corresponding Author
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
- Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
Keywords
double-strand DNA break, double-strand DNA break mapping, next generation sequencing, molecular biology, DNA damage, G-quadruplex, Transcription
Figure 1
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The authors declare no competing financial interests.
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Version 1 of Protocol Version 1 of Protocol Procedure steps 109 & 110 modified - Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
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Associated Publications
DSBCapture: in situ capture and sequencing of DNA breaks
by Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, +3
Nature Methods (15 August, 2016)
Method Article
Shankar Balasubramanian
Department of Chemistry, University of Cambridge
Corresponding Author
Version History
CURRENT STATUS: Posted
Version 1
Posted 23 Aug, 2016
No community comments so far
Metrics
Comments: 0
HTML Views: ...
License:
This work is licensed under a CC BY-NC 3.0 License. Read Full License
DSBCapture is a double-strand DNA break (DSB) mapping methodology that maps DSBs in situ to single-nucleotide resolution. DSBs are captured through the ligation of a modified Illumina adapter to broken DNA ends inside isolated, fixed nuclei. Break sites are subsequently decoded to single-nucleotide resolution by Illumina sequencing.
COMMENTS
Procedure steps 109 & 110 modified
- Ashleigh Carver, Editorial Assistant, Nature Protocols, 12/09/2016
Figure 1
Figure 2
Figure 3
Loading...
Associated Publications
DSBCapture: in situ capture and sequencing of DNA breaks
by Stefanie V Lensing, Giovanni Marsico, Robert Hänsel-Hertsch, +3
Nature Methods (15 August, 2016)