Isolation of murine hepatocytes to measure protein synthesis ex vivo upon stimulation

doi:10.1038/nprot.2008.209

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Method Article

Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Valentina Gandin

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy

Pier Carlo Marchisio

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; University Vita-Salute, Medical School, via Olgettina 58, 20132, Milan, Italy

Stefano Biffo

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; DISAV, University of Eastern Piedmont, via Bellini 8G, 15100, Alessandria, Italy

DOI:

10.1038/nprot.2008.209

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Associated Publications

Eukaryotic Initiation Factor 6 is rate-limiting in translation, growth and transformation
by Valentina Gandin, Annarita Miluzio, Anna Maria Barbieri, +4
Nature (11 August, 2008)

An open repository of community-contributed protocols sponsored by Nature Research.

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Method Article

Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Valentina Gandin

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy

Pier Carlo Marchisio

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; University Vita-Salute, Medical School, via Olgettina 58, 20132, Milan, Italy

Stefano Biffo

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; DISAV, University of Eastern Piedmont, via Bellini 8G, 15100, Alessandria, Italy

Version History

CURRENT STATUS: Posted

Version 1

Posted 29 Sep, 2008

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Subject Areas

Biochemistry

Computational biology and bioinformatics

DOI:

10.1038/nprot.2008.209

Download PDF

Associated Publications

Eukaryotic Initiation Factor 6 is rate-limiting in translation, growth and transformation
by Valentina Gandin, Annarita Miluzio, Anna Maria Barbieri, +4
Nature (11 August, 2008)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Valentina Gandin

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy

Pier Carlo Marchisio

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; University Vita-Salute, Medical School, via Olgettina 58, 20132, Milan, Italy

Stefano Biffo

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; DISAV, University of Eastern Piedmont, via Bellini 8G, 15100, Alessandria, Italy

DOI:

10.1038/nprot.2008.209

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Associated Publications

Eukaryotic Initiation Factor 6 is rate-limiting in translation, growth and transformation

by Valentina Gandin, Annarita Miluzio, Anna Maria Barbieri, +4

Nature (11 August, 2008)

An open repository of community-contributed protocols sponsored by Nature Research.

Learn more about Protocol Exchange

Method Article

Valentina Gandin, Pier Carlo Marchisio, Stefano Biffo

Valentina Gandin

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy

Pier Carlo Marchisio

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; University Vita-Salute, Medical School, via Olgettina 58, 20132, Milan, Italy

Stefano Biffo

Molecular Histology and Cell Growth Lab, San Raffaele Scientific Institute, via Olgettina 58, 20132, Milan, Italy; DISAV, University of Eastern Piedmont, via Bellini 8G, 15100, Alessandria, Italy

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DOI:

10.1038/nprot.2008.209

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Related Articles

Associated Publications

Eukaryotic Initiation Factor 6 is rate-limiting in translation, growth and transformation

by Valentina Gandin, Annarita Miluzio, Anna Maria Barbieri, +4

Nature (11 August, 2008)